仓鼠是否适合评估基于 RBD 的抗 COVID-19 亚单位疫苗的免疫原性？
Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?
机构信息
State Research Center of Virology and Biotechnology "Vector", Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia.
Russian-American Anti-Cancer Center, Altai State University, 656049 Barnaul, Russia.
出版信息
Viruses. 2022 May 16;14(5):1060. doi: 10.3390/v14051060.
Currently, SARS-CoV-2 spike receptor-binding-domain (RBD)-based vaccines are considered one of the most effective weapons against COVID-19. During the first step of assessing vaccine immunogenicity, a mouse model is often used. In this paper, we tested the use of five experimental animals (mice, hamsters, rabbits, ferrets, and chickens) for RBD immunogenicity assessments. The humoral immune response was evaluated by ELISA and virus-neutralization assays. The data obtained show hamsters to be the least suitable candidates for RBD immunogenicity testing and, hence, assessing the protective efficacy of RBD-based vaccines.
目前，基于严重急性呼吸综合征冠状病毒 2 刺突受体结合域（RBD）的疫苗被认为是对抗 COVID-19 的最有效武器之一。在评估疫苗免疫原性的第一步中，通常使用小鼠模型。在本文中，我们测试了五种实验动物（小鼠、仓鼠、兔子、雪貂和鸡）在 RBD 免疫原性评估中的应用。通过 ELISA 和病毒中和试验评估体液免疫反应。获得的数据表明仓鼠是 RBD 免疫原性测试和评估 RBD 疫苗保护效力的最不适宜候选者。
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