Zhang Yuan, Sheng Lei, Liu Xiao-Wei, Wei Juan, Liu Xiu-Juan, Zhang Nian-Yun, Wang Zi-Yi
School of Sports and Health, Nanjing Sport Institute, Nanjing 210014.
The Key Laboratory of Systems Biomedicine, Ministry of Education, and the Exercise Translational Medicine Centre, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2022 Jan;38(1):47-52. doi: 10.12047/j.cjap.6209.2022.009.
To investigate the effects of continuous exercise training (CT) and high-intensity interval exercise training (HIIT) on liver lipid metabolism and the correlation of the level of fibroblast growth factor 21(FGF21) in serum and liver tissues. Male SD rats were randomly divided into normal diet group (N) and obesity model group (H) after 1 week of adaptive feeding. Rats in the obesity model group were fed with 45% high-fat diet for about 8 weeks, and 20% weight increase compared with normal rats was considered as obesity. The rats were divided into normal diet control group (LC), normal diet HIIT group (LHI), normal diet CT group (LCT), High fat diet-induced obese control group (OC), obese HIIT group (OHI), and obese CT group (OCT) (=10). Exercised rats were given weight-bearing swimming training intervention for 8 weeks. Blood samples were collected at least 24h after the last exercise intervention to detect the serum levels of inflammatory factors and FGF21. Liver tissue samples were collected to detect the lipid content, lipid metabolic enzyme content and FGF21 expression level. Compared with LC group, the body weight, serum inflammatory factors levels and hepatic triglyceride content were increased significantly (＜0.05). Hepatic triglyceride content was downregulated in LHI group and FGF21 expression level was enhanced in LCT group (＜0.05). Compared with OC group, the body weight and hepatic triglyceride content were decreased significantly (＜0.05), mitochondrial CPT-1β and β-HAD enzyme contents in liver were increased significantly (＜0.05) in OHI group, the contents of LPL and FAT/CD36 enzyme in liver and the levels of FGF21 in serum and liver of OCT group were increased significantly (＜0.05). Both exercise modes can reduce the body weight in normal and obese rats, and lipid deposition in the liver of obese rats. HIIT has a more significant effect on alleviating liver lipid deposition in obese rats by upregulating mitochondrial lipid oxidation level in normal and obese rats. CT improves the levels of FGF21 in serum and liver tissues of normal and obese rats, enhances enzyme contents that involved in fatty acids uptake to the liver, which has limited effect on alleviating lipid deposition in liver of obese rats.
探讨持续运动训练（CT）和高强度间歇运动训练（HIIT）对肝脏脂质代谢的影响以及血清和肝脏组织中成纤维细胞生长因子21（FGF21）水平的相关性。雄性SD大鼠适应性喂养1周后，随机分为正常饮食组（N）和肥胖模型组（H）。肥胖模型组大鼠给予45%高脂饮食约8周，体重较正常大鼠增加20%视为肥胖。将大鼠分为正常饮食对照组（LC）、正常饮食HIIT组（LHI）、正常饮食CT组（LCT）、高脂饮食诱导肥胖对照组（OC）、肥胖HIIT组（OHI）和肥胖CT组（OCT）（每组n = 10）。运动大鼠进行8周的负重游泳训练干预。在最后一次运动干预后至少24小时采集血样，检测血清炎症因子和FGF21水平。采集肝脏组织样本，检测脂质含量、脂质代谢酶含量和FGF21表达水平。与LC组相比，体重、血清炎症因子水平和肝脏甘油三酯含量显著升高（P＜0.05）。LHI组肝脏甘油三酯含量下调，LCT组FGF21表达水平增强（P＜0.05）。与OC组相比，OHI组体重和肝脏甘油三酯含量显著降低（P＜0.05），肝脏线粒体CPT-1β和β-HAD酶含量显著增加（P＜0.05），OCT组肝脏LPL和FAT/CD36酶含量以及血清和肝脏中FGF21水平显著增加（P＜0.05）。两种运动模式均可降低正常和肥胖大鼠的体重以及肥胖大鼠肝脏中的脂质沉积。HIIT通过上调正常和肥胖大鼠的线粒体脂质氧化水平，对减轻肥胖大鼠肝脏脂质沉积有更显著的作用。CT提高了正常和肥胖大鼠血清和肝脏组织中FGF21的水平，增强了参与脂肪酸摄取到肝脏的酶含量，对减轻肥胖大鼠肝脏脂质沉积的作用有限。
