Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China.
Department of Ophthalmology, Binzhou Medical University Hospital, Binzhou, Shandong, China.
Curr Eye Res. 2022 Sep;47(9):1266-1271. doi: 10.1080/02713683.2022.2079143. Epub 2022 May 30.
This study aimed to explore whether corneal cross-linking (CXL) could regress corneal blood vessels (CBV) and corneal lymphatic vessels (CLV) in alkali-burned rabbits.
A total of 80 rabbits 2-3 months old weighing 1.5-2.0 kg were randomly divided into four groups: CXL7 group; CTL7 group; CXL14 group; and CTL14 group. Then, 3% sodium pentobarbital 1 ml/kg and tetracaine eye drop 5 g/L were administered before surgery. NaOH 2 mol/L was topically applied to the central cornea to establish the alkali burning model. Then CXL was administered within 2 h in groups CXL7 and CXL14. Corneal opacity and edema, CBV and CLV volume, cluster differentiation 31 (CD31), and lymphatic vessel endothelial receptor 1 (LYVE-1) expression levels were analyzed on days 7 and 14.
CXL reduced cornea opacity, CNV, and CLV volumes on day 7 in alkali-burned rabbits. However, CNV and CLV volumes were increased on day 14. CXL also showed down- and upregulation of CD31 and LYVE-1 expression levels on days 7 and 14, respectively.
CXL effectively regulated CBV and CLV in alkali-burned rabbits. The transient angioregression and lymphangioregression induced by CXL may be potentially helpful in vascularized high-risk eyes.
本研究旨在探讨角膜交联（CXL）是否能使碱烧伤兔的角膜血管（CBV）和角膜淋巴管（CLV）消退。
将 80 只 2-3 月龄、体重 1.5-2.0kg 的兔子随机分为 4 组：CXL7 组、CTL7 组、CXL14 组和 CTL14 组。然后，在手术前用 1ml/kg 的 3%戊巴比妥钠和 5g/L 地卡因滴眼。用 2mol/L NaOH 局部涂于角膜中央建立碱烧伤模型。然后，在 CXL7 和 CXL14 组中于 2 小时内进行 CXL。分析第 7 天和第 14 天的角膜混浊和水肿、CBV 和 CLV 体积、分化簇 31（CD31）和淋巴管内皮受体 1（LYVE-1）的表达水平。
CXL 降低了碱烧伤兔第 7 天的角膜混浊、CNV 和 CLV 体积。然而，第 14 天 CNV 和 CLV 体积增加。CXL 还分别在第 7 天和第 14 天显示 CD31 和 LYVE-1 表达水平的下调和上调。
CXL 有效地调节了碱烧伤兔的 CBV 和 CLV。CXL 诱导的短暂血管消退和淋巴管消退可能对血管化高危眼有潜在的帮助。
