Metabolic Platform, Biochemistry Laboratory, Faculty of Medicine, Cadi Ayad, University, Marrakech, Morocco.
Neurosciences, Pharmacology and Environment Unit (NPEU), Faculty of Sciences, Semlalia, Cadi Ayyad University, Marrakech, Morocco.
Pharm Biol. 2022 Dec;60(1):879-888. doi: 10.1080/13880209.2022.2068618.
Chondroitin 6 sulphate (C6S) is a glycosaminoglycan (GAG) whose accumulation is notable in mucopolysaccharidosis type IVA and VII. Flaxseed, L. (Linaceae) (FS), is reported to have comparable properties to those of soybean, a source of genistein, a potential new treatment for MPSs.
We assess the effect of total ethanol flaxseed extract (EFSE) in an animal model of C6S accumulation.
The study was performed in adult male Wistar rats ( = 24) for 15 successive days. The animals were divided into four groups: (1) control injected with physiological saline buffer, (2) intoxicated rats injected intraperitoneally with C6S, (3) intoxicated with C6S and treated with EFSE, and (4) treated with EFSE. All groups were subjected to histopathological and biochemical studies. The antioxidant and phytochemical properties of EFSE were examined.
Dry EFSE contains total phenols (6.28 mg EAG/g), condensed tannins (2.98 mg ECAT/g) and flavonoids (0.44 mg ECAT/g) with high antioxidant potential [RPE (IC = 8.37 ± 0.176), DPPH (IC = 12.79 ± 0.273)]. The LD is higher than 5000 mg/kg. The histopathological examination showed an accumulation of C6S in the C6S intoxicated group, which disappeared in the C6S-EFSE treated group. GAGs assays showed an increased excretion in the C6S intoxicated group and increased excretion of 14% in the C6S-EFSE group compared to the C6S group.
EFSE showed significant potential for chelation. Its use for the treatment of GAG accumulation could be suggested and generalized to a larger study population.
硫酸软骨素 6 盐（C6S）是一种糖胺聚糖（GAG），在黏多糖贮积症 IVA 型和 VII 型中积累明显。亚麻籽，亚麻科（亚麻科）（FS），据报道具有与大豆相当的特性，大豆是染料木黄酮的来源，染料木黄酮是 MPSs 的一种潜在新疗法。
我们评估总乙醇亚麻籽提取物（EFSE）在 C6S 积累动物模型中的作用。
这项研究在 24 只成年雄性 Wistar 大鼠中进行了 15 天的连续研究。将动物分为四组：（1）对照组注射生理盐水缓冲液，（2）腹腔注射 C6S 的中毒大鼠，（3）用 EFSE 治疗 C6S 中毒的大鼠，（4）用 EFSE 治疗的大鼠。所有组均进行组织病理学和生化研究。检查 EFSE 的抗氧化和植物化学特性。
干 EFSE 含有总酚（6.28mg EAG/g）、缩合单宁（2.98mg ECAT/g）和类黄酮（0.44mg ECAT/g），具有很高的抗氧化潜力[RPE（IC = 8.37±0.176），DPPH（IC = 12.79±0.273）]。LD 大于 5000mg/kg。组织病理学检查显示 C6S 中毒组 C6S 蓄积，C6S-EFSE 治疗组蓄积消失。GAG 测定显示 C6S 中毒组排泄增加，C6S-EFSE 组比 C6S 组排泄增加 14%。
EFSE 显示出显著的螯合潜力。其用于治疗 GAG 蓄积的用途可以被提出并推广到更大的研究人群。
