Department of Obstetrics, Hengshui Fourth People's Hospital, Hengshui, Hebei, China.
Department of Clinical Pharmacy, The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei, China.
Immun Inflamm Dis. 2022 Jun;10(6):e624. doi: 10.1002/iid3.624.
This study used astragalus membranaceus (AM) to treat systemic lupus erythematosus (SLE) model mice during pregnancy, aiming to explore the role of AM in Helper T cell 17 (Th17) differentiation and SLE during pregnancy.
We used lipopolysaccharide to constructed the SLE mouse model. AM decoction given by intragastric administration lasted from the eighth week of the mouse age until the mouse was killed. We estimated the messenger RNA levels of IL-17a and Rorc, counted the Th17 cell number and examined the levels of cytokines including interleukin (IL)-12, tumor necrosis factor α, interferon gamma, IL-17A in mouse serum. Periodic acid-Schiff staining and renal glomerular/tubulointerstitial (TI) score were used to evaluate the progression of lupus nephritis (LN).
AM treatment improved the conception rate and increased the number and average weight of fetuses in SLE mice. It significantly decreased the urinary albumin/creatinine ratios and reduced the glomerular scores and TI scores in the pregnant SLE mice. AM gavage significantly decreased the weight of spleen, mesenteric lymph node, total splenocytes and T cells, and the expression of proinflammatory factors. Furthermore, AM treatment reduced the ratio of Th17 cells and the expression levels of RORγt and IL-17A.
AM significantly improved pregnancy outcomes and inhibited lupus nephritis during pregnancy in SLE mice.
本研究采用黄芪（AM）治疗妊娠系统性红斑狼疮（SLE）模型小鼠，旨在探讨 AM 在辅助性 T 细胞 17（Th17）分化和妊娠期间 SLE 中的作用。
我们使用脂多糖构建 SLE 小鼠模型。AM 煎剂通过灌胃给药，从第 8 周龄持续到处死小鼠。我们估计了 IL-17a 和 Rorc 的信使 RNA 水平，计数了 Th17 细胞数量，并检测了包括白细胞介素（IL）-12、肿瘤坏死因子 α、干扰素 γ、IL-17A 在内的细胞因子在小鼠血清中的水平。过碘酸希夫染色和肾肾小球/肾小管间质（TI）评分用于评估狼疮肾炎（LN）的进展。
AM 治疗改善了 SLE 小鼠的受孕率，增加了胎儿的数量和平均体重。它显著降低了尿白蛋白/肌酐比值，并降低了妊娠 SLE 小鼠的肾小球评分和 TI 评分。AM 灌胃显著降低了脾、肠系膜淋巴结、总脾细胞和 T 细胞的重量，以及促炎因子的表达。此外，AM 治疗降低了 Th17 细胞的比例和 RORγt 和 IL-17A 的表达水平。
AM 显著改善了 SLE 小鼠的妊娠结局，并抑制了妊娠期间的狼疮肾炎。
