Nosek T M, Fender K Y, Godt R E
Science. 1987 Apr 10;236(4798):191-3. doi: 10.1126/science.3563496.
The increases in the intracellular concentrations of inorganic phosphate and hydrogen ion accompanying fatigue of skeletal muscle appear to be the most important metabolic changes associated with the decrease in contractile force. Experiments on chemically skinned single fibers from rabbit psoas muscle with pH ranging between 6 and 7.25 demonstrate that the depression of maximal calcium-activated force by inorganic phosphate correlates nicely with the concentration of the acidic (diprotonated) species. Therefore, in addition to the well-known depressant effect on the contractile machinery of lowering pH per se, any decrease of intracellular pH associated with fatigue further depresses force production by converting more of the total inorganic phosphate within the cell to the inhibitory diprotonated form.
骨骼肌疲劳时细胞内无机磷酸盐和氢离子浓度的升高似乎是与收缩力下降相关的最重要的代谢变化。对兔腰大肌化学去膜单纤维进行的实验，pH值在6至7.25之间，结果表明无机磷酸盐对最大钙激活力的抑制作用与酸性（双质子化）物质的浓度密切相关。因此，除了众所周知的降低pH本身对收缩机制的抑制作用外，与疲劳相关的细胞内pH的任何降低都会通过将细胞内更多的总无机磷酸盐转化为抑制性双质子化形式，进一步抑制力的产生。
