Clinic of Small Animal Surgery and Reproduction at the Centre for Clinical Veterinary Medicine, Faculty of Veterinary Medicine, LMU Munich, Munich, Germany.
Chair of Anatomy, Histology and Embryology, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Munich, Germany.
J Feline Med Surg. 2022 Aug;24(8):e168-e174. doi: 10.1177/1098612X221099195. Epub 2022 May 30.
Ovarian remnant syndrome (ORS) is suspected when heat signs occur in spayed individuals, but further diagnostic procedures are necessary to exclude other possible oestrogen sources, such as the adrenal gland or exogenous supplementation. Anti-Müllerian hormone (AMH), secreted by granulosa cells or Sertoli cells, serves to differentiate sexually intact from gonadectomised animals and has been described in dogs as a tool for diagnosing ORS. The aim of this study was to evaluate if AMH determination can be used to diagnose ORS in cats.
AMH was measured with a chemiluminescence immunoassay in serum samples of 15 sexually intact, 9 spayed and 16 cats with a history of heat signs after spaying. Abdominal ultrasound (n = 13), vaginal smears (n = 7), progesterone measurement (n = 5) and laparotomy (n = 14) were used to determine the presence of ovarian tissue. After surgery, a histological examination of the obtained tissue was performed in the cats with suspected ORS.
In 15 cats with ORS the AMH serum concentrations were significantly higher than in spayed cats (n = 10; = 0.025) and significantly lower than in sexually intact cats (n = 15; = 0.001). Among the cats with ORS, the highest AMH serum concentrations were measured in the queens with cystic ovarian alterations and in one cat from which a whole ovary was obtained. The cat with the lowest AMH serum concentration had a simultaneous high progesterone serum concentration. Cats with ORS did not show any heat signs after surgical removal of the ovarian tissue.
A single determination of AMH in blood serum is a useful diagnostic tool for the diagnosis of ORS in cats, regardless of the hormonal activity of the remnant ovarian tissue.
在绝育个体中出现发热迹象时，怀疑存在卵巢残余综合征（ORS），但需要进一步的诊断程序来排除其他可能的雌激素来源，如肾上腺或外源性补充。抗缪勒管激素（AMH）由颗粒细胞或支持细胞分泌，用于区分未去势和去势动物，并已在犬中被描述为诊断 ORS 的工具。本研究旨在评估 AMH 测定是否可用于诊断猫的 ORS。
采用化学发光免疫分析法测定 15 只未去势、9 只绝育和 16 只绝育后有发情迹象的猫的血清 AMH 水平。对 13 只猫进行腹部超声检查、7 只猫进行阴道涂片检查、5 只猫进行孕激素测量、14 只猫进行剖腹探查，以确定卵巢组织的存在。在疑似 ORS 的猫中进行手术后，对获得的组织进行组织学检查。
在 15 只 ORS 猫中，AMH 血清浓度明显高于绝育猫（n=10； =0.025），明显低于未去势猫（n=15； =0.001）。在 ORS 猫中，卵巢囊性改变的母猫和获得整个卵巢的一只母猫的 AMH 血清浓度最高。AMH 血清浓度最低的猫同时具有较高的孕激素血清浓度。在切除卵巢组织后，ORS 猫均未出现发情迹象。
单次测定血清 AMH 是诊断猫 ORS 的有用诊断工具，与残余卵巢组织的激素活性无关。
