College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
Jiuquan City Animal Control Disease Center, Jiuquan, China.
Pol J Microbiol. 2022 Mar 30;71(1):63-71. doi: 10.33073/pjm-2022-009.
In order to clarified characteristics and function of internalin G (inlG) in ATCC19111 (1/2a) (LM), the immune protection of the inlG was evaluated in mice, the homologous recombination was used to construct deletion strains, and their biological characteristics were studied by the transcriptomics analysis. As a result, the immunization of mice with the purified protein achieved a protective effect against bacterial infection. The deletion strain LM-AinlG was successfully constructed with genetic stability. The mouse infection test showed that the virulence of LM was decreased after the deletion of the gene. The deletion strain showed enhanced adhesion to and invasion of Caco-2 cells. Compared to the wild strain, 18 genes were up-regulated, and 24 genes were down-regulated in the LM-AinlG. This study has laid a foundation for further research on the function of and the pathogenesis of LM. In this study, immunization of mice with the purified inlG protein achieved a protective effect against infection. The virulence of LM-ΔinlG was decreased by mouse infection. However, the adhesion and invasion ability to Caco-2 cell were enhanced. Compared to the wild strain, 18 genes were up-regulated, and 24 genes were down-regulated in the LM-ΔinlG. This study has laid a foundation for further study of the function of the inlG and the listeriosis.
为了阐明 ATCC19111(1/2a)(LM) 中内毒素 G(inlG)的特征和功能，评估了 inlG 在小鼠中的免疫保护作用，通过同源重组构建了缺失株，并通过转录组分析研究了它们的生物学特性。结果表明，用纯化蛋白免疫小鼠可获得针对细菌感染的保护作用。成功构建了遗传稳定性良好的缺失株 LM-AinlG。小鼠感染试验表明，该基因缺失后 LM 的毒力降低。缺失株对 Caco-2 细胞的黏附和侵袭能力增强。与野生株相比，LM-AinlG 中有 18 个基因上调，24 个基因下调。本研究为进一步研究 功能和 LM 的发病机制奠定了基础。本研究中，用纯化的 inlG 蛋白免疫小鼠可获得针对 感染的保护作用。LM-ΔinlG 的毒力通过小鼠感染降低。然而，对 Caco-2 细胞的黏附和侵袭能力增强。与野生株相比，LM-ΔinlG 中有 18 个基因上调，24 个基因下调。本研究为进一步研究 inlG 的功能和李斯特菌病奠定了基础。
