Department of Chemistry, BITS, Pilani K K Birla Goa Campus, NH 17B Bypass Road, Zuarinagar, Goa 403726, India.
Department of Biological Sciences, BITS, Pilani K K Birla Goa Campus, NH 17B Bypass Road, Zuarinagar, Goa 403726, India.
Analyst. 2022 Jun 27;147(13):2997-3006. doi: 10.1039/d2an00040g.
A coumarin coupled tetraphenylethylene based AIEgen (TPE-Lac) with an intense greenish-yellow emission has been synthesized and utilized for multipurpose sensing and imaging applications. TPE-Lac acts as a sensitive sensor for the detection of cyanide ions (CN) with an immediate turn-off response in the presence of many other interfering cations and anions. The limit of detection (LOD) was as low as 33 nM, which is well below the permissible limit set by the World Health Organization (WHO). Cyanide detection in the solid phase was successfully demonstrated by drop-casting the solution of the TPE-Lac probe on TLC plates and measuring and analysing the fluorescence response by ImageJ analysis. TPE-Lac was further employed in the detection of explosive nitroaromatics in solution and solid phases. Also, TPE-Lac was found suitable as an imaging agent and could easily percolate into live H520 cells giving bright fluorescence from the intra-cellular region. Easy and cost-effective synthesis, fast response and low LODs are some of the advantages of this AIEgen over available molecular probes for the same purpose.
一种香豆素偶联的基于四苯乙烯的聚集诱导发光（AIE）化合物（TPE-Lac）具有强烈的绿黄色发射，已被合成并用于多种传感和成像应用。TPE-Lac 可以作为一种灵敏的传感器，用于检测氰根离子（CN），在存在许多其他干扰阳离子和阴离子的情况下，具有立即关闭的响应。检测限（LOD）低至 33 nM，远低于世界卫生组织（WHO）规定的允许限值。通过将 TPE-Lac 探针的溶液滴在 TLC 板上，并使用 ImageJ 分析测量和分析荧光响应，成功地在固相中进行了氰化物检测。TPE-Lac 进一步用于溶液和固相中爆炸型硝基芳烃的检测。此外，TPE-Lac 被发现适合作为成像剂，并且可以很容易地渗透到活的 H520 细胞中，从细胞内区域发出明亮的荧光。与用于相同目的的现有分子探针相比，这种 AIE 化合物具有易于合成、响应速度快和检测限低等优点。
