杨梅素可改善SAMP8小鼠的认知功能，并上调脑源性神经营养因子和神经生长因子。
Myricetin improves cognitive function in SAMP8 mice and upregulates brain-derived neurotrophic factor and nerve growth factor.
作者信息
Shimada Yu, Sato Yuka, Kumazoe Motofumi, Kitamura Ryo, Fujimura Yoshinori, Tachibana Hirofumi
机构信息
Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan.
Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan.
出版信息
Biochem Biophys Res Commun. 2022 Aug 6;616:33-40. doi: 10.1016/j.bbrc.2022.05.039. Epub 2022 May 20.
INTRODUCTION
Considering that neurodegeneration is an irreversible process, an efficient, low-burden approach to prevent dementia is strongly needed. Here, we show that the daily intake of myricetin normalised cognitive dysfunction in senescence-accelerated mouse prone 8 (SAMP8) mice.
METHODS
SAMP8 mice were fed a diet supplemented with myricetin and novel object recognition tests and Y-maze tests were performed. Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in brains of SAMP8 mice were measured. The phosphorylation level of cAMP-response-element-binding protein (CREB) level in brains of SAMP8 mice were evaluated. Also, SH-SY5Y cells were treated with myricetin and cAMP levels were measured.
RESULTS
In SAMP8 mice, neurotrophins, including BDNF and NGF, were downregulated relative to levels in their normal counterparts. In addition, myricetin intake upregulated the phosphorylation of CREB, the major transcription factor for BDNF and NGF. Also, myricetin induced cAMP upregulation, and CREB phosphorylation via a cAMP-dependent protein kinase-dependent manner in SH-SY5Y cells.
CONCLUSION
Taken together, myricetin improves cognitive function in SAMP8 mice and upregulates BDNF and NGF.
引言
鉴于神经退行性变是一个不可逆的过程，迫切需要一种高效、低负担的方法来预防痴呆症。在此，我们表明，每日摄入杨梅素可使衰老加速小鼠8型（SAMP8）的认知功能障碍恢复正常。
方法
给SAMP8小鼠喂食添加了杨梅素的饮食，并进行新物体识别测试和Y迷宫测试。测量SAMP8小鼠大脑中的脑源性神经营养因子（BDNF）和神经生长因子（NGF）。评估SAMP8小鼠大脑中cAMP反应元件结合蛋白（CREB）的磷酸化水平。此外，用杨梅素处理SH-SY5Y细胞并测量cAMP水平。
结果
在SAMP8小鼠中，与正常对照相比，包括BDNF和NGF在内的神经营养因子下调。此外，摄入杨梅素可上调CREB的磷酸化，CREB是BDNF和NGF的主要转录因子。此外，杨梅素在SH-SY5Y细胞中通过cAMP依赖性蛋白激酶依赖性方式诱导cAMP上调和CREB磷酸化。
结论
综上所述，杨梅素可改善SAMP8小鼠的认知功能，并上调BDNF和NGF。
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