Álvarez-García Samuel, Gutiérrez Santiago, Casquero Pedro A
Grupo Universitario de Investigación en Ingeniería y Agricultura Sostenible (GUIIAS), Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de León, Avenida Portugal 41, 24071 León, Spain.
Grupo Universitario de Investigación en Ingeniería y Agricultura Sostenible (GUIIAS), Área de Microbiología, Escuela de Ingeniería Agraria y Forestal, Universidad de León, Campus de Ponferrada, Avenida Astorga s/n, 24401 Ponferrada, Spain.
MethodsX. 2022 May 18;9:101734. doi: 10.1016/j.mex.2022.101734. eCollection 2022.
Although increasing focus is being given to the control of insect pests with natural products, the study of volatile mediated microbe-insect interactions has been largely neglected so far. Currently, no standardized method is available for the evaluation of the effects produced on insects by Volatile Organic Compounds (VOCs) directly emitted by actively growing microbial strains. This manuscript presents a reliable protocol using VOC Chambers (Álvarez-García et al., 2021) to assess these effects and specifically those exerted on dry grain insect pests. The methodology also serves to evaluate the impact of these volatile interactions on the grain damage produced by the insects. It allows to vary the level of gas exchange with the environment, thus providing different experimental conditions to emulate diverse natural ones in which these interactions may occur. Our method adapts the use of VOC Chambers for the evaluation of microbe-insect volatile interactions and the screening of microbial strains that produce potentially bioactive VOCs against insects. It allows assessing the effects of these volatile interactions on the damage produced by insect pests on stored dry grains. Sealed and unsealed setups can be used to determine the impact of ventilation and gas exchange on these interactions.
尽管人们越来越关注利用天然产物控制害虫，但迄今为止，挥发性介导的微生物与昆虫相互作用的研究在很大程度上被忽视了。目前，尚无标准化方法可用于评估活跃生长的微生物菌株直接排放的挥发性有机化合物（VOCs）对昆虫产生的影响。本手稿介绍了一种使用VOC室（Álvarez-García等人，2021年）的可靠方案，以评估这些影响，特别是对干谷物害虫的影响。该方法还用于评估这些挥发性相互作用对昆虫造成的谷物损害的影响。它可以改变与环境的气体交换水平，从而提供不同的实验条件，以模拟这些相互作用可能发生的各种自然条件。我们的方法适用于使用VOC室评估微生物与昆虫的挥发性相互作用，以及筛选对昆虫产生潜在生物活性VOCs的微生物菌株。它可以评估这些挥发性相互作用对储存在干谷物中的害虫造成的损害的影响。密封和未密封的设置可用于确定通风和气体交换对这些相互作用的影响。
