Huzhou Key Laboratory of Medical and Environmental Applications Technologies, School of Life Sciences, Huzhou University, Zhejiang, 313000, P.R. China.
Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Bioengineering, University of Science and Technology Beijing, Beijing 100083, P.R. China.
Analyst. 2022 Jun 13;147(12):2633-2639. doi: 10.1039/d2an00562j.
Nowadays, test strips are widely applied, but their use is mostly limited to the qualitative or half-quantitative analysis of targets. The main reason for their limited use is the "Coffee Ring Effect" (CRE) of probe materials, which leads to a heterogeneous probe distribution and poor testing reproducibility and sensitivity. In the present work, a fluorescent test strip was fabricated with a suppressed CRE of silver nanocluster (AgNC) probes coated by gelatin (Gel) under vacuum-aided fast lyophilization. Uniform and stable deposition of AgNC probes was achieved onto the test strips with a high loading capacity. The AgNCs displayed specific responses to Hg ions, allowing sensitive and quantitative analysis in the linear concentration ranges from 0.20 to 50000 nM with a limit of detection of 0.10 nM. Given the advantages of rapid and facile preparation, CRE suppression, high biocompatibility, and cost-effectiveness, such a fabrication protocol may pave the way for the design of various test strips-based devices for point-of-care analytical applications in the fields of environmental monitoring, food quality analysis, and clinical diagnostics.
如今，测试条被广泛应用，但它们的用途大多局限于目标的定性或半定量分析。其使用受限的主要原因是探针材料的“咖啡环效应”（CRE），这导致了探针分布不均匀，测试重现性和灵敏度较差。在本工作中，通过在真空辅助快速冻干下用明胶（Gel）涂覆银纳米团簇（AgNC）探针，制备了具有抑制 CRE 的荧光测试条。AgNC 探针均匀且稳定地沉积在测试条上，具有高负载能力。AgNC 对 Hg 离子表现出特异性响应，可在 0.20 至 50000 nM 的线性浓度范围内进行灵敏和定量分析，检测限为 0.10 nM。鉴于其快速简便的制备、CRE 抑制、高生物相容性和成本效益的优势，这种制备方案可能为基于测试条的各种设备的设计铺平道路，可用于环境监测、食品质量分析和临床诊断等领域的即时分析应用。
