Aursnes I, Sundal J, Nome T
Thromb Res. 1987 Jan 1;45(1):29-37. doi: 10.1016/0049-3848(87)90254-4.
Platelet rich plasma was prepared with soybean trypsin inhibitor (SBTI) as anticoagulant, to preserve normal Ca++-concentration, and subsequently tested in an aggregometer. Shear stress alone, induced by stirring, resulted in platelet aggregation and disaggregation. Such platelets showed only minor aggregational response on the addition of ADP, indicating that the platelets had been partly desensitized towards ADP-stimulation. When the stirrer was started at the same time as the addition of 1.6 microM ADP, the aggregation velocity was related to the speed of the stirrer. Disaggregation always started 1.5 min after the stimulus. Platelet shape change was apparently not influenced by the stirring. Since platelets from SBTI-anticoagulated blood could be aggregated by stirring alone, we examined the effect of centrifugation of whole blood at 50 xg for 3 and 10 min. Signs of transient aggregation and release of alpha-granule content were respectively observed.
用大豆胰蛋白酶抑制剂（SBTI）作为抗凝剂制备富含血小板血浆，以保持正常的Ca++浓度，随后在血小板聚集仪中进行检测。仅通过搅拌诱导的剪切应力会导致血小板聚集和解聚。这种血小板在添加ADP时仅表现出轻微的聚集反应，表明血小板已对ADP刺激部分脱敏。当搅拌器与添加1.6 microM ADP同时启动时，聚集速度与搅拌器速度相关。解聚总是在刺激后1.5分钟开始。搅拌显然不影响血小板形状变化。由于来自SBTI抗凝血液的血小板仅通过搅拌就能聚集，我们研究了全血在50 xg下离心3分钟和10分钟的效果。分别观察到了短暂聚集和α颗粒内容物释放的迹象。
