加蓬咝蝰（Bitis gabonica）毒液中存在组胺的证据。

Evidence for the presence of histamine in Gaboon viper (Bitis gabonica) venom.

作者信息

Tilmisany A K, Mustafa A A, Abdel Aziz A, Osman O H

出版信息

Toxicon. 1986;24(11-12):1159-61. doi: 10.1016/0041-0101(86)90142-x.

Abstract

Venom from B. gabonica produced a dose-dependent contraction of both isolated guinea-pig ileum and rabbit aortic strip preparations. The venom-induced contraction was not antagonised by pretreatment with atropine, phentolamine, methysergide or indomethacin, however, it was blocked by the prior addition of chlorpheniramine or cyproheptadine. Upon exhaustive dialysis of the venom, the dialysate contracted both preparations, while the dialysed venom had no effect. Paper and thin-layer chromatography of the venom showed a spot with an Rf value corresponding to authentic histamine. Biological assay of the crude venom on the guinea-pig ileum showed that it contained the equivalent of 2-10 micrograms of histamine per mg dry weight of crude venom. pA2 values for chlorpheniramine using either the venom or histamine as agonists were not significantly different.

摘要

加蓬咝蝰的毒液能使分离出的豚鼠回肠和兔主动脉条标本产生剂量依赖性收缩。然而，用阿托品、酚妥拉明、甲基麦角新碱或吲哚美辛预处理不能拮抗毒液诱导的收缩，但预先加入氯苯那敏或赛庚啶可阻断该收缩。对毒液进行彻底透析后，透析液能使两种标本收缩，而经过透析的毒液则无作用。毒液的纸色谱和薄层层析显示有一个Rf值与纯组胺相对应的斑点。粗毒液对豚鼠回肠的生物测定表明，每毫克粗毒液干重中含相当于2 - 10微克组胺的量。以毒液或组胺作为激动剂时，氯苯那敏的pA2值无显著差异。