National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, China.
Sci Total Environ. 2022 Sep 15;839:156330. doi: 10.1016/j.scitotenv.2022.156330. Epub 2022 May 28.
Knowledge gaps still surround the question of what biofilms form on contaminated microplastics (MPs) in the antibiotics and (or) heavy metals polluted sewage. In this work, the clean polyethylene microplastics (PE MPs) and triclocarban (TCC)-contaminated PE MPs were cultured in the sewage containing only ampicillin (AMP), only copper (Cu) and both AMP and Cu for 28 days. The results showed that the TCC on PE MPs (with concentration of 2.48 mg/g PE MPs) did not impede the adhesion of the bacteria and the formation of biofilm. Moreover, many potential pathogenic bacteria (Aquabacterium and Pseudoxanthomonas) and potential resistant bacteria (Stenotrophomonas) were more likely to attach on TCC-contaminated PE MPs compared with clean PE MPs. In addition, biofilms of TCC-contaminated PE MPs had highest potential pathogenic functions. TCC-contaminated PE MPs also caused the increases of various resistance genes in both biofilm and sewage. The co-occurrence of TCC, AMP and Cu might exert a stronger selective pressure on bacterial communities and promote the co-selection of resistance genes. In addition, TCC-contaminated PE MPs resulted in higher abundance of five mobile genetic elements (MGEs) (intI1, intI3, tnpA-04, IS613 and trb-C) in sewage, which might further promote the transmission of resistance genes.
关于受抗生素和/或重金属污染污水中污染物在污染的微塑料（MPs）上形成生物膜的问题，仍存在知识空白。在这项工作中，将干净的聚乙烯微塑料（PE MPs）和三氯卡班（TCC）污染的 PE MPs 在仅含有氨苄西林（AMP）、仅含铜（Cu）以及同时含有 AMP 和 Cu 的污水中培养 28 天。结果表明，PE MPs 上的 TCC（浓度为 2.48 mg/g PE MPs）并没有阻碍细菌的附着和生物膜的形成。此外，与干净的 PE MPs 相比，许多潜在的病原菌（Aquabacterium 和 Pseudoxanthomonas）和潜在的耐药菌（Stenotrophomonas）更容易附着在 TCC 污染的 PE MPs 上。此外，TCC 污染的 PE MPs 的生物膜具有最高的潜在致病功能。TCC 污染的 PE MPs 还导致生物膜和污水中各种耐药基因的增加。TCC、AMP 和 Cu 的共同存在可能对细菌群落施加更强的选择压力，并促进耐药基因的共同选择。此外，TCC 污染的 PE MPs 导致污水中五种移动遗传元件（MGEs）（intI1、intI3、tnpA-04、IS613 和 trb-C）的丰度更高，这可能进一步促进耐药基因的传播。
