Department of Plant Breeding, Justus Liebig University, Giessen, Germany.
Methods Mol Biol. 2022;2481:287-310. doi: 10.1007/978-1-0716-2237-7_17.
Array-based SNP markers are commonly used in genome-wide association studies (GWAS) to identify genomic regions involved in important agronomical traits. However, conversion of these SNP markers into breeder-friendly kompetitive allele-specific PCR (KASP) markers for use in marker-assisted selection is often challenging. In this chapter we describe general considerations and successfully applied protocols for the conversion of Illumina array SNP markers into locus-specific KASP markers with a special emphasis and examples on how to overcome difficulties in polyploid wheat.
基于阵列的 SNP 标记通常用于全基因组关联研究 (GWAS) 来鉴定与重要农艺性状相关的基因组区域。然而，将这些 SNP 标记转换为适合育种者使用的竞争等位基因特异性 PCR (KASP) 标记，以便用于标记辅助选择，通常具有挑战性。在本章中，我们描述了将 Illumina 阵列 SNP 标记转换为特定于基因座的 KASP 标记的一般考虑因素和成功应用的方案，并特别强调和举例说明了如何克服在多倍体小麦中遇到的困难。
