miR-30 通过靶向 MTA1 抑制骨肉瘤的进展。
miR-30 inhibits the progression of osteosarcoma by targeting MTA1.
机构信息
Department of Ultrasonic Medicine, Affiliated Hospital of Inner Mongolia Medical University, China.
Health Center, Ningbo customhouse, China.
出版信息
J Musculoskelet Neuronal Interact. 2022 Jun 1;22(2):261-268.
OBJECTIVES
MicroRNAs (miRNAs) have been considered as a new class of novel diagnostic and predictive biomarker in many diseases. However, there are few studies on miRNA in osteosarcoma (OS). This study aimed to investigate the roles of miR-30 on OS occurrence and development.
METHODS
PCR was used to detect mRNA levels of miR-30 and MTA1 in cancer tissues, adjacent non-cancerous tissues from OS patients. Western blot was used to detect MTA1 protein expression in all tissues and cell lines (hFOb1.19,Saos-2, MG63, and U2OS). The correlation between miR-30 and MTA1 was predicted through bioinformatics software, and identified by a luciferase reporting experiment. In vitro, functional test detected the specific effects of miR-30 and MTA1 on the development of OS.
RESULTS
miR-30 expression was significantly reduced, while the expression of MTA1 was increased in OS tissues and cells. Luciferase reporting experiment showed that miR-30 sponged MTA1 which was negatively correlated with miR-30 expression. Furthermore, rescue tests revealed that MTA1 restrained the functions of miR-30 on cell proliferation and migration of OS.
CONCLUSION
Our finding showed that miR-30 modulated the proliferation and migration by targeting MTA1 in OS.
目的
微小 RNA（miRNAs）已被认为是许多疾病中新型诊断和预测生物标志物的新类别。然而，关于骨肉瘤（OS）中 miRNA 的研究较少。本研究旨在探讨 miR-30 在 OS 发生和发展中的作用。
方法
采用 PCR 检测骨肉瘤患者癌组织、癌旁非癌组织中 miR-30 和 MTA1 的 mRNA 水平。Western blot 检测所有组织和细胞系（hFOb1.19、Saos-2、MG63 和 U2OS）中 MTA1 蛋白的表达。通过生物信息学软件预测 miR-30 和 MTA1 之间的相关性，并通过荧光素酶报告实验进行鉴定。在体外，功能试验检测了 miR-30 和 MTA1 对 OS 发展的特定影响。
结果
miR-30 在 OS 组织和细胞中的表达明显降低，而 MTA1 的表达增加。荧光素酶报告实验表明，miR-30 可吸附与 miR-30 表达呈负相关的 MTA1。此外，挽救试验表明，MTA1 抑制了 miR-30 对 OS 细胞增殖和迁移的功能。
结论
我们的研究结果表明，miR-30 通过靶向 OS 中的 MTA1 来调节细胞增殖和迁移。
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