Balan Alkora Ioana, Halaţiu Vasile Bogdan, Comșulea Emilian, Mutu Cosmin Constantin, Cozac Dan Alexandru, Aspru Ioana, Păcurar Delia, Bănescu Claudia, Perian Marcel, Scridon Alina
Physiology Department, University of Medicine, Pharmacy, Science and Technology "George Emil Palade", 540139 Târgu Mureș, Romania.
Cardiology Department, Emergency Institute for Cardiovascular Diseases and Transplantation, 540139 Târgu Mureș, Romania.
Int J Mol Sci. 2025 Mar 26;26(7):3049. doi: 10.3390/ijms26073049.
Using an atrial fibrillation (AF) model in spontaneously hypertensive rats (SHRs), we aimed to identify circulating miRNAs for AF diagnosis and prediction and to confirm the cardiac origin of these miRNAs. A total of 31 SHRs and 39 Wistar Kyoto (WKY) normotensive controls were randomized into six groups: young, adult, and aging SHR and WKY. Spontaneous AF burden and atrial and circulating levels of 11 miRNAs were quantified. Spontaneous AF was absent in all WKY rats. In the SHRs, AF episodes were observed in two adult animals and in all aging animals (13.6 ± 2.3 episodes/24 h). The atrial levels of five miRNAs were significantly higher in adult and aging SHRs compared to their WKY controls (all < 0.05). Of these, only the circulating levels of miR-328 were significantly higher in the aging SHRs vs. WKYs ( < 0.0001). Atrial miR-328 levels in the SHRs increased progressively with age ( < 0.001) and correlated with circulating miR-328 levels (r = 0.58; < 0.01). Among aging SHRs, atrial levels of miR-328 strongly correlated with AF burden (r = 0.79; < 0.01). These data suggest that the circulating level of miR-328 could emerge as a promising marker for both AF diagnosis and, if assessed dynamically, for AF prediction.
我们使用自发性高血压大鼠（SHR）的心房颤动（AF）模型，旨在鉴定用于AF诊断和预测的循环miRNA，并确认这些miRNA的心脏来源。总共31只SHR和39只Wistar Kyoto（WKY）正常血压对照大鼠被随机分为六组：年轻、成年和老龄SHR组以及WKY组。对11种miRNA的自发性AF负荷以及心房和循环水平进行了定量分析。所有WKY大鼠均未出现自发性AF。在SHR中，两只成年动物和所有老龄动物均观察到AF发作（13.6±2.3次发作/24小时）。与WKY对照相比，成年和老龄SHR中五种miRNA的心房水平显著更高（均P<0.05）。其中，只有老龄SHR中miR-328的循环水平显著高于WKY组（P<0.0001）。SHR中心房miR-328水平随年龄增长而逐渐升高（P<0.001），并与循环miR-328水平相关（r = 0.58；P<0.01）。在老龄SHR中，miR-328的心房水平与AF负荷密切相关（r = 0.79；P<0.01）。这些数据表明，miR-328的循环水平可能成为AF诊断以及动态评估时AF预测的一个有前景的标志物。
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