Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, 310016, China.
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, International Research Center for X Polymers, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
Biomater Sci. 2022 Jun 28;10(13):3612-3623. doi: 10.1039/d2bm00536k.
Drug-coated balloons (DCBs) offer potential to deliver drugs to treat coronary lesions but without leaving permanent implants behind. Paclitaxel and sirolimus are anti-proliferation drugs that are commonly used in commercially available DCBs. However, these drugs present significant cytotoxicity concern and low efficacy . Here, we use microRNA-22 (miR-22) as balloon loaded drugs and polyelectrolyte complexes (PECs) polyethyleneimine/polyacrylic acid (PEI/PAA) as balloon coatings to establish a new DCB system through the ultrasonic spray method. The PEI/PAA forms a stable and thin coating on the balloon, which resulted in a good transfer capacity to the vessel wall both and . miR-22 that could modulate smooth muscle cell (SMC) phenotype switching is incorporated into the PEI/PAA coating and shows a sustained release profile. The PEI/PAA/miR-22 coated balloon successfully inhibits intima hyperplasia after balloon-induced vascular injury in a rat model through decreasing proliferative SMCs the miR-22-methyl-CpG binding protein 2 (MECP2) axis. Our findings indicate that balloons coated with PEI/PAA/miR-22 have great potential to be promising DCBs in the treatment of cardiovascular disease.
载药球囊（DCBs）为将药物递送至治疗冠状动脉病变提供了潜力，而不会留下永久性植入物。紫杉醇和西罗莫司是常用于市售 DCBs 的抗增殖药物。然而，这些药物存在显著的细胞毒性问题和低疗效。在这里，我们使用 microRNA-22 (miR-22) 作为球囊负载药物，聚电解质复合物（PECs）聚乙烯亚胺/聚丙烯酸（PEI/PAA）作为球囊涂层，通过超声喷雾法建立了一种新的 DCB 系统。PEI/PAA 在球囊上形成稳定且薄的涂层，这导致其对血管壁具有良好的传递能力。可以调节平滑肌细胞（SMC）表型转换的 miR-22 被整合到 PEI/PAA 涂层中，并表现出持续释放的特性。PEI/PAA/miR-22 涂层球囊通过降低增殖性 SMCs 和 miR-22-甲基-CpG 结合蛋白 2 (MECP2) 轴，成功抑制了大鼠模型中球囊诱导的血管损伤后的内膜增生。我们的研究结果表明，PEI/PAA/miR-22 涂层的球囊在治疗心血管疾病方面具有很大的潜力，有望成为有前途的 DCBs。
