Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Key Laboratory of Aquatic Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Institute of Hydrobiology, Jinan University, West 601 Huangpu Avenue, Tianhe District, Guangzhou 510632, People's Republic of China.
Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Key Laboratory of Aquatic Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Institute of Hydrobiology, Jinan University, West 601 Huangpu Avenue, Tianhe District, Guangzhou 510632, People's Republic of China.
Vet Parasitol. 2022 Jun;306:109731. doi: 10.1016/j.vetpar.2022.109731. Epub 2022 May 22.
Ichthyophthirius multifiliis, a global distributed protozoan parasite, causes "White spot disease" and leads to serious mortality of freshwater fish in aquaculture. The present study was conducted to assess the anti-I. multifiliis efficacy of active compound isolated from Sophora flavescens. The isolated active compound was identified as sophoraflavanone G (SG) with ESI-MS and NMR. In vitro tests, SG at concentrations of 0.5 mg/L and 2 mg/L resulted in death of all theronts and tomonts, respectively; SG at concentrations of 0.125 mg/L and 0.25 mg/L notably decreased theronts infectivity (p < 0.05). Additionally, the in vivo test results showed that a cumulative delivery of SG at concentration of 2 mg/L for 7 days protected fish from I. multifiliis infection. The 96-h LC (median lethal concentration) and safety concentration of SG to grass carp were 46.6 mg/L and 11.3 mg/L, respectively. The present work indicated that SG was a potential safe and effectively therapeutic agent in treating I. multifiliis.
多子小瓜虫，一种广泛分布的原生动物寄生虫，会引起“白点病”，导致水产养殖中淡水鱼的严重死亡。本研究旨在评估从苦参中分离得到的活性化合物对多子小瓜虫的抗虫效果。分离得到的活性化合物被鉴定为槐属黄酮 G（SG），通过 ESI-MS 和 NMR 进行鉴定。体外试验结果表明，浓度为 0.5mg/L 和 2mg/L 的 SG 分别导致所有的滋养体和包囊体死亡；浓度为 0.125mg/L 和 0.25mg/L 的 SG 显著降低了滋养体的感染力（p<0.05）。此外，体内试验结果表明，连续 7 天以 2mg/L 的浓度投送苦参碱可保护鱼类免受多子小瓜虫感染。SG 对草鱼的 96-h LC50（半数致死浓度）和安全浓度分别为 46.6mg/L 和 11.3mg/L。本研究表明，SG 是一种治疗多子小瓜虫的潜在安全有效治疗药物。
