Parma A E, Fernández A S, Santisteban C G, Bowden R A, Cerone S I
Vet Immunol Immunopathol. 1987 Feb;14(2):181-5. doi: 10.1016/0165-2427(87)90052-3.
An antigenic relationship between Leptospira interrogans and equine cornea was previously described by us. An enzyme-linked immunosorbent assay was employed in the present work to investigate the existence of anti-leptospira and anti-cornea antibodies in tears, aqueous humor and serum from horses inoculated i.m. with those antigens. Ten days after a booster by the same route, antibodies that bind to microtiter plates, coated with an homogenate of either equine cornea or leptospira, were detected in those fluids and in the sera. At the same time, the corneas of the horses began to develop a diffuse opacity. This finding of anti-leptospira antibodies in equine tears and aqueous humor shows the pathway along which they arrive at the cornea and bind to it.
我们之前曾描述过问号钩端螺旋体与马角膜之间的抗原关系。在本研究中，我们采用酶联免疫吸附测定法，调查经肌肉注射这些抗原的马的眼泪、房水和血清中抗钩端螺旋体抗体和抗角膜抗体的存在情况。在通过相同途径进行加强免疫10天后，在这些液体和血清中检测到了与包被有马角膜或钩端螺旋体匀浆的微量滴定板结合的抗体。与此同时，马的角膜开始出现弥漫性混浊。在马的眼泪和房水中发现抗钩端螺旋体抗体，这表明了它们到达角膜并与之结合的途径。
