Chen Sen-Min, Liu Si-Xi, Chen Fen, Wang Chun-Yan, Mai Hui-Rong, Yuan Xiu-Li, Wen Fei-Qiu
Department of Hematology and Oncology, Shenzhen Children's Hospital, Shenzhen, Guangdong 518038, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2022 May 15;24(5):550-560. doi: 10.7499/j.issn.1008-8830.2110045.
To examine the changes of intestinal flora in children newly diagnosed with acute lymphoblastic leukemia (ALL) and the influence of chemotherapy on intestinal flora.
Fecal samples were collected from 40 children newly diagnosed with ALL before chemotherapy and at 2 weeks, 1 month, and 2 months after chemotherapy. Ten healthy children served as the control group. 16S rDNA sequencing and analysis were performed to compare the differences in intestinal flora between the ALL and control groups and children with ALL before and after chemotherapy.
The ALL group had a significant reduction in the abundance of intestinal flora at 1 and 2 months after chemotherapy, with a significant reduction compared with the control group (<0.05). Compared with the control group, the ALL group had a significant reduction in the diversity of intestinal flora before and after chemotherapy (<0.05). At the phylum level, compared with the control group, the ALL group had a significant reduction in the relative abundance of at 2 weeks, 1 month, and 2 months after chemotherapy (<0.05) and a significant increase in the relative abundance of at 1 and 2 months after chemotherapy (<0.05). At the genus level, compared with the control group, the ALL group had a significant reduction in the relative abundance of at 2 weeks, 1 month, and 2 months after chemotherapy (<0.05); the relative abundance of in the ALL group was significantly higher than that in the control group at 1 and 2 months after chemotherapy and showed a significant increase at 1 month after chemotherapy (<0.05); the relative abundance of in the ALL group was significantly lower than that in the control group before and after chemotherapy and showed a significant reduction at 2 weeks and 1 month after chemotherapy (<0.05). The relative abundance of increased significantly at 1 and 2 months after chemotherapy in the ALL group (<0.05), and was significantly higher than that in the control group (<0.05).
The diversity of intestinal flora in children with ALL is significantly lower than that in healthy children. Chemotherapy significantly reduces the abundance of intestinal flora and can reduce the abundance of some probiotic bacteria ( and ) and increase the abundance of pathogenic bacteria ( and ) in children with ALL.
探讨新诊断急性淋巴细胞白血病（ALL）患儿肠道菌群的变化以及化疗对肠道菌群的影响。
收集40例新诊断ALL患儿化疗前及化疗后2周、1个月、2个月的粪便样本。选取10例健康儿童作为对照组。采用16S rDNA测序分析比较ALL组与对照组以及ALL患儿化疗前后肠道菌群的差异。
ALL组化疗后1个月和2个月肠道菌群丰度显著降低，与对照组相比差异有统计学意义（<0.05）。与对照组相比，ALL组化疗前后肠道菌群多样性显著降低（<0.05）。在门水平上，与对照组相比，ALL组化疗后2周、1个月和2个月 相对丰度显著降低（<0.05），化疗后1个月和2个月 相对丰度显著升高（<0.05）。在属水平上，与对照组相比，ALL组化疗后2周、1个月和2个月 相对丰度显著降低（<0.05）；ALL组化疗后1个月和2个月 相对丰度显著高于对照组，且化疗后1个月显著升高（<0.05）；ALL组化疗前后 相对丰度显著低于对照组，化疗后2周和1个月显著降低（<0.05）。ALL组化疗后1个月和2个月 相对丰度显著升高（<0.05），且显著高于对照组（<0.05）。
ALL患儿肠道菌群多样性显著低于健康儿童。化疗显著降低肠道菌群丰度，可降低ALL患儿部分益生菌（ 和 ）的丰度，增加病原菌（ 和 ）的丰度。
