Department of Veterinary Medicine, University of Santa Catarina State, Lages, Santa Catarina, Brazil.
Laboratory of Bacterial Zoonoses, Department of Preventive Veterinary Medicine and Animal Health, Cidade Universitária Armando de Salles Oliveira, Butantã, São Paulo, SP, Brazil.
Top Companion Anim Med. 2022 Sep-Oct;50:100672. doi: 10.1016/j.tcam.2022.100672. Epub 2022 May 27.
This report describes the clinical features and molecular diagnosis of a case of canine leproid granuloma (CLG) caused by mycobacterial strains of the Mycobacterium simiae complex in Brazil. A 12-year-old non-neutered male Labrador Retriever dog was presented with a 2-week history of progressive painless cutaneous lesions. Ulcerated nodules with hematic crusts were observed on the dorsal surface of the right and left pinna and on the metacarpal, metatarsal, and digits. Complete blood count, serum biochemistry, aspiration cytology of cutaneous lesions, biopsy for histopathological evaluation, culture for aerobic and anaerobic bacteria, polymerase chain reaction and DNA sequencing to identify mycobacterial species were performed. According to the clinical and histopathological findings, a diagnosis of CLG was established. Despite the negative result of the bacterial culture, mycobacterial identification was made by sequencing the hsp65 gene. Our findings highlight that mycobacterial species closely related to members of the M simiae clade can be causative agents of CLG.
本报告描述了巴西一株猿猴分枝杆菌复合群分枝杆菌引起犬麻风样肉芽肿（CLG）的临床特征和分子诊断。一只 12 岁、未去势的雄性拉布拉多猎犬因渐进性无痛性皮肤损伤就诊，病程 2 周。右侧和左侧耳廓以及掌骨、跖骨和指骨背表面可见溃疡性结节，伴有血性痂皮。进行了全血细胞计数、血清生化、皮肤病变抽吸细胞学检查、用于组织病理学评估的活检、需氧和厌氧菌培养、聚合酶链反应和 DNA 测序以鉴定分枝杆菌物种。根据临床和组织病理学发现，诊断为 CLG。尽管细菌培养结果为阴性，但通过 hsp65 基因测序确定了分枝杆菌的鉴定。我们的研究结果表明，与猿猴分枝杆菌群成员密切相关的分枝杆菌物种可能是 CLG 的致病因子。
