Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong.
Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong.
Pathology. 2022 Oct;54(6):721-728. doi: 10.1016/j.pathol.2022.03.003. Epub 2022 May 26.
The use of immunostain for PRAME antigen is well established for cutaneous melanolocytic lesions. However, its staining in other cutaneous structures and lesions is under reported. This study assessed PRAME staining in a large cohort of normal skin tissue, sebaceous lesions, and cutaneous carcinomas to better delineate patterns of PRAME immunoreactivity. PRAME immunostaining was performed on sections of sebaceous lesions and tissue microarrays of basal cell carcinomas (BCCs) and squamous cell carcinomas (SCCs). Normal cutaneous adnexal structures were assessed on the sections of sebaceous lesions. For sebaceous lesions and non-lesional sebaceous glands, PRAME immunostaining was assessed for mature, germinative and sebocytes independently. A total of 193 sebaceous lesions, 64 BCCs and 35 SCCs were stained for PRAME immunostain. Staining pattern was predominantly cytoplasmic in normal apocrine glands, germinative sebocytes of sebaceous glands, and hair germs (p<0.001). Lesional sebocytes did not show different staining compared to normal sebaceous glands (p>0.05). Rare nuclear staining was observed in the normal epidermis (0.6%) and junctional melanocytes (4.1%). BCC, SCC and sebaceous carcinoma all showed low levels of PRAME immunoreactivity with variable proportions of cases demonstrating nuclear staining (BCC 59.4%, SCC 37.1%, sebaceous carcinoma 5.3%). PRAME immunostaining is positive in germinative sebocytes, various cutaneous structures and carcinomas. Nuclear staining, identical to melanoma, was observed in normal epidermis, junctional melanocytes, BCCs, SCCs, and sebaceous carcinomas. The pattern of PRAME staining in the skin must be recognised to avoid pitfalls in interpretating PRAME immunostain.
PRAME 抗原的免疫染色在皮肤黑色素细胞病变中得到了很好的应用。然而，其在其他皮肤结构和病变中的染色报道较少。本研究评估了 PRAME 在一大组正常皮肤组织、皮脂腺病变和皮肤癌中的染色情况，以更好地描绘 PRAME 免疫反应的模式。对皮脂腺病变和组织微阵列的基底细胞癌 (BCC) 和鳞状细胞癌 (SCC) 进行了 PRAME 免疫染色。在皮脂腺病变的切片上评估了正常皮肤附属器结构。对于皮脂腺病变和非病变性皮脂腺，分别评估了 PRAME 免疫染色的成熟、生殖和皮脂腺细胞。共对 193 例皮脂腺病变、64 例 BCC 和 35 例 SCC 进行了 PRAME 免疫染色。染色模式在正常大汗腺、皮脂腺的生殖性皮脂腺细胞和毛球中主要为细胞质 (p<0.001)。病变性皮脂腺细胞与正常皮脂腺细胞的染色无差异 (p>0.05)。在正常表皮 (0.6%) 和交界性黑素细胞 (4.1%) 中观察到罕见的核染色。BCC、SCC 和皮脂腺癌均显示出低水平的 PRAME 免疫反应性，不同比例的病例显示核染色 (BCC 59.4%、SCC 37.1%、皮脂腺癌 5.3%)。PRAME 免疫染色在生殖性皮脂腺细胞、各种皮肤结构和癌中呈阳性。与黑色素瘤相同的核染色在正常表皮、交界性黑素细胞、BCC、SCC 和皮脂腺癌中均有观察到。在解读 PRAME 免疫染色时，必须识别皮肤中 PRAME 染色的模式，以避免出现陷阱。
