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本文引用的文献

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PMP2/FABP8 induces PI(4,5)P-dependent transbilayer reorganization of sphingomyelin in the plasma membrane.PMP2/FABP8 诱导质膜中鞘磷脂的 PI(4,5)P 依赖性跨膜重排。
Cell Rep. 2021 Nov 9;37(6):109935. doi: 10.1016/j.celrep.2021.109935.
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Cellular cholesterol and how to find it.细胞胆固醇及其检测方法
Biochim Biophys Acta Mol Cell Biol Lipids. 2021 Sep;1866(9):158989. doi: 10.1016/j.bbalip.2021.158989. Epub 2021 Jun 10.
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Imaging cholesterol depletion at the plasma membrane by methyl-β-cyclodextrin.通过甲基-β-环糊精对质膜上胆固醇消耗进行成像。
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The use of pore-forming toxins to image lipids and lipid domains.利用成孔毒素对脂类和脂域进行成像。
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Disturbances in PP2A methylation and one-carbon metabolism compromise Fyn distribution, neuritogenesis, and APP regulation.PP2A 甲基化和一碳代谢紊乱会损害 Fyn 的分布、轴突生成和 APP 调节。
J Biol Chem. 2021 Jan-Jun;296:100237. doi: 10.1074/jbc.RA120.016069. Epub 2021 Jan 7.
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Cholesterol access in cellular membranes controls Hedgehog signaling.胆固醇进入细胞膜控制 Hedgehog 信号通路。
Nat Chem Biol. 2020 Dec;16(12):1303-1313. doi: 10.1038/s41589-020-00678-2. Epub 2020 Nov 16.
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A Key Motif in the Cholesterol-Dependent Cytolysins Reveals a Large Family of Related Proteins.胆固醇依赖性细胞溶素中的关键模体揭示了一大类相关蛋白。
mBio. 2020 Sep 29;11(5):e02351-20. doi: 10.1128/mBio.02351-20.
8
Binding specificity of ostreolysin A6 towards Sf9 insect cell lipids.扇贝溶素 A6 对 Sf9 昆虫细胞脂质的结合特异性。
Biochim Biophys Acta Biomembr. 2020 Sep 1;1862(9):183307. doi: 10.1016/j.bbamem.2020.183307. Epub 2020 Apr 13.
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Cholesterol asymmetry at the tip of filopodia during cell adhesion.细胞黏附过程中丝状伪足尖端的胆固醇不对称性。
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Intracellular cholesterol trafficking is dependent upon NPC2 interaction with lysobisphosphatidic acid.细胞内胆固醇转运依赖于 NPC2 与溶血磷脂酸的相互作用。
Elife. 2019 Oct 3;8:e50832. doi: 10.7554/eLife.50832.

一种新型固醇结合蛋白揭示了神经突生长和晚期内体/溶酶体中胆固醇的不均匀分布。

A novel sterol-binding protein reveals heterogeneous cholesterol distribution in neurite outgrowth and in late endosomes/lysosomes.

机构信息

Lipid Biology Laboratory, RIKEN, 2-1, Hirosawa, Wako-shi, Saitama, 351-0198, Japan.

Cellular Informatics Laboratory, RIKEN CPR, 2-1, Hirosawa, Wako-shi, Saitama, 351-0198, Japan.

出版信息

Cell Mol Life Sci. 2022 May 29;79(6):324. doi: 10.1007/s00018-022-04339-6.

DOI:10.1007/s00018-022-04339-6
PMID:35644822
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11072113/
Abstract

We identified a mushroom-derived protein, maistero-2 that specifically binds 3-hydroxy sterol including cholesterol (Chol). Maistero-2 bound lipid mixture in Chol-dependent manner with a binding threshold of around 30%. Changing lipid composition did not significantly affect the threshold concentration. EGFP-maistero-2 labeled cell surface and intracellular organelle Chol with higher sensitivity than that of well-established Chol probe, D4 fragment of perfringolysin O. EGFP-maistero-2 revealed increase of cell surface Chol during neurite outgrowth and heterogeneous Chol distribution between CD63-positive and LAMP1-positive late endosomes/lysosomes. The absence of strictly conserved Thr-Leu pair present in Chol-dependent cytolysins suggests a distinct Chol-binding mechanism for maistero-2.

摘要

我们鉴定了一种蘑菇衍生的蛋白质，mastero-2，它特异性地结合 3-羟基固醇，包括胆固醇（Chol）。Mastore-2 以 Chol 依赖性方式结合脂质混合物，结合阈值约为 30%。改变脂质组成并不显著影响阈值浓度。与已建立的 Chol 探针，产气荚膜梭菌的 perfringolysin O 的 D4 片段相比，EGFP-maistero-2 更灵敏地标记细胞膜表面和细胞内细胞器 Chol。在神经突生长过程中，EGFP-maistero-2 显示细胞膜表面 Chol 增加，并且 CD63 阳性和 LAMP1 阳性晚期内体/溶酶体之间存在 Chol 分布不均。在 Chol 依赖性细胞溶素中不存在严格保守的 Thr-Leu 对表明，mastero-2 具有独特的 Chol 结合机制。