Tan Haitao, Huang Shan, Wang Tingrui
Department of Spinal Surgery, Hainan Province Clinical Medical Center, The First Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570102, China.
Department of Cardiology, The First Affiliated Hospital of Hainan Medical University, Haikou 570102, China.
Evid Based Complement Alternat Med. 2022 May 18;2022:6540557. doi: 10.1155/2022/6540557. eCollection 2022.
To evaluate the clinical significance of peripheral blood T helper cell 1 (Th1) and T helper cell 17 (Th17) cell content and serum (interleukin) IL-35 and IL-17 expression in patients with ankylosing spondylitis (AS).
In this retrospective study, we included and assigned 60 cases of AS patients enrolled in our hospital from January 2019 to January 2020 to an active group (ankylosing spondylitis disease activity score (ASDAS) ≥2.1, = 28) and a static group (ASDAS<2.1, = 32) according to the degree of disease activity. The logistic propensity score matching method was used to include 60 healthy individuals after a physical examination during the same period in the control group. The peripheral blood Th1 and Th17 cell content and the levels of serum IL-35 and IL-17 were determined and analyzed.
Statistically significant differences were found in the Th1 cell ratio and Th17 cell ratio between the control group and the other two groups ( < 0.05), and the static group yielded a higher Th1 cell ratio and a lower Th17 cell ratio than the active group ( < 0.05). Statistically significant differences were also observed in the serum IL-35 and IL-17 levels between the control group and the other two groups ( < 0.05), and the static group had a higher IL-35 level and a lower IL-17 level than the active group ( < 0.05).
The imbalance of Th17/Th1 cell content of AS patients is characterized by high expression of IL-17 and low expression of IL-35. The increased activity of AS was associated with a dominant state of Th17 cells and a significant increase in IL-17 expression, indicating that Treg/Th17 imbalance is closely related to the development of AS, which may provide new ideas for the prevention and treatment of AS.
评估强直性脊柱炎（AS）患者外周血辅助性T细胞1（Th1）和辅助性T细胞17（Th17）细胞含量以及血清白细胞介素（IL）-35和IL-17表达的临床意义。
在这项回顾性研究中，我们纳入了2019年1月至2020年1月在我院就诊的60例AS患者，并根据疾病活动程度将其分为活动组（强直性脊柱炎疾病活动评分（ASDAS）≥2.1，n = 28）和静止组（ASDAS<2.1，n = 32）。采用逻辑倾向评分匹配法纳入同期体检合格的60名健康个体作为对照组。检测并分析外周血Th1和Th17细胞含量以及血清IL-35和IL-17水平。
对照组与其他两组之间Th1细胞比例和Th17细胞比例存在统计学显著差异（P<0.05），静止组的Th1细胞比例高于活动组，Th17细胞比例低于活动组（P<0.05）。对照组与其他两组之间血清IL-35和IL-17水平也存在统计学显著差异（P<0.05），静止组的IL-35水平高于活动组，IL-17水平低于活动组（P<0.05）。
AS患者Th17/Th1细胞含量失衡表现为IL-17高表达和IL-35低表达。AS活动度增加与Th17细胞的优势状态以及IL-17表达显著增加有关，表明Treg/Th17失衡与AS的发生发展密切相关，这可能为AS的防治提供新思路。
