Asmamaw Mengstie Misganaw
Department of Biochemistry, College of Medicine and Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia.
Front Bioeng Biotechnol. 2022 May 12;10:895713. doi: 10.3389/fbioe.2022.895713. eCollection 2022.
The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) and its accompanying protein (Cas9) are now the most effective, efficient, and precise genome editing techniques. Two essential components of the CRISPR/Cas9 system are guide RNA (gRNA) and CRISPR-associated (Cas9) proteins. Choosing and implementing safe and effective delivery systems in the therapeutic application of CRISPR/Cas9 has proven to be a significant problem. For CRISPR/Cas9 delivery, viral vectors are the natural specialists. Due to their higher delivery effectiveness than other delivery methods, vectors such as adenoviral vectors (AdVs), adeno-associated viruses (AAVs), and lentivirus vectors (LVs) are now commonly employed as delivery methods. This review thoroughly examined recent achievements in using a variety of viral vectors as a means of CRISPR/Cas9 delivery, as well as the benefits and limitations of each viral vector. Future thoughts for overcoming the current restrictions and adapting the technology are also discussed.
成簇规律间隔短回文重复序列（CRISPR）及其相关蛋白（Cas9）是目前最有效、高效且精确的基因组编辑技术。CRISPR/Cas9系统的两个关键组件是向导RNA（gRNA）和CRISPR相关（Cas9）蛋白。在CRISPR/Cas9的治疗应用中选择并实施安全有效的递送系统已被证明是一个重大问题。对于CRISPR/Cas9递送而言，病毒载体是天生的行家。由于其递送效率高于其他递送方法，腺病毒载体（AdV）、腺相关病毒（AAV）和慢病毒载体（LV）等载体目前通常被用作递送手段。本综述全面研究了使用多种病毒载体作为CRISPR/Cas9递送手段的最新成果，以及每种病毒载体的优缺点。还讨论了克服当前限制并改进该技术的未来思路。
