Moungprayoon Ayyapruk, Lunprom Siriporn, Reungsang Alissara, Salakkam Apilak
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand.
Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen, Thailand.
Front Bioeng Biotechnol. 2022 May 12;10:878688. doi: 10.3389/fbioe.2022.878688. eCollection 2022.
High cell density cultivation is a promising approach to reduce capital and operating costs of poly (3-hydroxybutyrate) (PHB) production. To achieve high cell concentration, it is necessary that the cultivation conditions are adjusted and controlled to support the best growth of the PHB producer. In the present study, carbon to nitrogen (C/N) ratio of a sugarcane juice (SJ)-based medium, initial sugar concentration, and dissolved oxygen (DO) set point, were optimized for batch cultivation of sp. KKU01. A maximum biomass concentration of 55.5 g/L was attained using the C/N ratio of 10, initial sugar concentration of 100 g/L, and 20% DO set point. Fed-batch cultivation conducted under these optimum conditions, with two feedings of SJ-based medium, gave the final cell concentration of 87.9 g/L, with a PHB content, concentration, and yield of 36.2%, 32.1 g/L, and 0.13 g/g-sugar, respectively. A medium-based economic analysis showed that the economic yield of PHB on nutrients was 0.14. These results reveal the possibility of using SJ for high cell density cultivation of sp. KKU01 for PHB production. However, further optimization of the process is necessary to make it more efficient and cost-effective.
高细胞密度培养是一种有望降低聚（3-羟基丁酸酯）（PHB）生产成本和运营成本的方法。为了实现高细胞浓度，有必要调整和控制培养条件，以支持PHB生产者的最佳生长。在本研究中，针对sp. KKU01的分批培养，对基于甘蔗汁（SJ）的培养基的碳氮（C/N）比、初始糖浓度和溶解氧（DO）设定点进行了优化。使用C/N比为10、初始糖浓度为100 g/L和20%的DO设定点，获得了55.5 g/L的最大生物量浓度。在这些最佳条件下进行补料分批培养，分两次补加基于SJ的培养基，最终细胞浓度为87.9 g/L，PHB含量、浓度和产量分别为36.2%、32.1 g/L和0.13 g/g-糖。基于培养基的经济分析表明，PHB对营养物质的经济产量为0.14。这些结果揭示了使用SJ进行sp. KKU01高细胞密度培养以生产PHB的可能性。然而，有必要进一步优化该工艺，以使其更高效且更具成本效益。
