Department of Microbiology, Government College University, Faisalabad, Pakistan.
Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, Pakistan.
Biomed Res Int. 2022 May 18;2022:5816986. doi: 10.1155/2022/5816986. eCollection 2022.
causes tuberculosis in dairy and wild animals. Presence of tuberculosis in animals poses a threat not only to their herd mates but also for public. No reports are available about the clinical, pathological, and molecular investigation of naturally occurring tuberculosis (TB) due to in one-horned rhinoceros. One-horned female rhinoceros () at the age of 41 years died in a public park in Pakistan. Postmortem and other investigations were carried out to know the cause of death. The present study describes necropsy, histopathology, and molecular-based confirmation of TB in a captive female rhinoceros that died of this infection. Clinically, the rhinoceros showed nonspecific clinical signs including anorexia, lethargy, dyspnoea, coughing, and sudden death. At necropsy, the trachea exhibited mild congestion and contained catarrhal exudate at the bronchial bifurcation. Macroscopic examination revealed characteristic tubercles on all parenchymatous organs. The lungs showed consolidation, grey hepatization, and contained granulomatous lesions packed with cheesy exudate. Histopathological examination showed severe pneumonic changes in the form of granulomatous inflammation consisting of lymphocytes, multinucleated giant cells, caseous materials, and mineralized foci surrounded by a fibrous capsule. PCR amplicon of 500 bp confirmed the presence of in multiple hepatic and pulmonary tissue samples, as well as in uterine exudates. It was concluded that the presence of tuberculosis in rhinoceros may pose potential transmission risk to other animals and the application of practical tools to determine TB status in the rhinoceros is crucial.
导致奶牛和野生动物患结核病。动物结核病的存在不仅对它们的同类构成威胁，也对公众构成威胁。由于独角犀牛，目前尚无关于自然发生的结核病 (TB) 的临床、病理学和分子调查的报告。一只 41 岁的雌性独角犀牛 () 在巴基斯坦的一个公共公园中死亡。进行了尸检和其他调查以了解死亡原因。本研究描述了一头因感染而死亡的圈养雌性犀牛的剖检、组织病理学和基于分子的结核病确认。临床上，犀牛表现出非特异性临床症状，包括食欲不振、嗜睡、呼吸困难、咳嗽和突然死亡。尸检时，气管显示轻度充血，支气管分叉处有卡他性渗出物。肉眼检查显示所有实质器官上都有特征性的结核结节。肺部呈现出实变、灰色肝化，并含有充满奶酪样渗出物的肉芽肿病变。组织病理学检查显示严重的肺炎性变化，形成由淋巴细胞、多核巨细胞、干酪样物质和矿化灶组成的肉芽肿性炎症，周围有纤维囊。500bp 的 PCR 扩增物证实了在多个肝和肺组织样本以及子宫分泌物中存在 。结论是，犀牛中结核病的存在可能对其他动物构成潜在的传播风险，应用实用工具来确定犀牛中的结核病状况至关重要。
