Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, Pakistan.
Sciences and Research, College of Nursing, Umm Al Qura University, 715 Makkah, Saudi Arabia.
Biomed Res Int. 2022 Jun 2;2022:7601463. doi: 10.1155/2022/7601463. eCollection 2022.
() being the main cause of animal tuberculosis is a complex infectious agent and can be a cause of zoonotic tuberculosis zoonosis in public health. To date, the uncommon infection in public health due to still is a great challenge to both veterinary and medical professions and requires a careful diagnosis and confirmation of the bacterium. Therefore, this study for the first time reports the clinical, gross, histopathological, and molecular based confirmation of infection in wildlife animals (nilgai). Prior to death, the morbid animal showed severe pneumonic ailments like moist cough, thick nasal exudates, and dyspnoea. At necropsy, enlargement of mandibular cervical and mesenteric lymph nodes was observed. Different macroscopic lesions such as congestion and hyperaemia, creamy white and catarrhal exudates in trachea, consolidation, grey and red hepatisation of lungs, and micro- and macrogranulomatous tubercles containing caseous materials in lungs were observed. The heart of morbid animal showed congestions, myocarditis, and a copious amount of straw-colored fluid in the pericardial sac. At the microscopic level, lungs indicated granulomatous inflammatory response, presence of multinucleated giant cells, fibrosis, and punctuation of alveoli with chronic inflammatory cells. Histopathological examination of various sections of the heart of the infected animal showed chronic inflammatory response consisting of chronic inflammatory cells like monocyte, lymphocytes, and fibroblasts along with noncalcified eosinophilic materials. At the molecular level, infection was confirmed in various tissues like the heart, lungs, cervical, and mesenteric lymph nodes in morbid animals. In conclusion, based on our results, it can be suggested that more molecular based epidemiological studies are crucial to know the exact cause of pulmonary and cervical tuberculosis in wild animals.
（）是动物结核病的主要原因，是一种复杂的感染因子，可在公共卫生领域引起人畜共患结核病。迄今为止，由于仍然罕见，这种感染在公共卫生领域对兽医和医学专业来说仍是一个巨大的挑战，需要仔细诊断和确认该细菌。因此，本研究首次报告了野生动物（印度野牛）中感染的临床、大体、组织病理学和分子学确认。在死亡之前，患病动物表现出严重的肺炎症状，如湿性咳嗽、浓稠的鼻腔分泌物和呼吸困难。在剖检时，观察到下颌颈和肠系膜淋巴结肿大。观察到不同的大体病变，如充血和充血、气管中的乳白色和卡他性渗出物、肺实变、肺的灰色和红色肝变以及含有干酪样物质的微和大肉芽肿性结核。患病动物的心脏表现出充血、心肌炎和心包囊中有大量稻草色液体。在显微镜下，肺显示出肉芽肿性炎症反应，存在多核巨细胞、纤维化和肺泡点状慢性炎症细胞。感染动物心脏各部位的组织病理学检查显示，存在慢性炎症反应，包括单核细胞、淋巴细胞和成纤维细胞等慢性炎症细胞以及未钙化的嗜酸性物质。在分子水平上，在患病动物的心脏、肺、颈和肠系膜淋巴结等各种组织中均确认了感染。总之，根据我们的结果，可以认为进行更多基于分子的流行病学研究对于了解野生动物肺部和颈部结核病的确切原因至关重要。
