直接浸入式固相微萃取结合环境电离串联质谱法，快速鉴别血清中的农药，用于紧急诊断。
Direct immersion solid-phase microextraction combined with ambient ionization tandem mass spectrometry to rapidly distinguish pesticides in serum for emergency diagnostics.
机构信息
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, Taiwan.
出版信息
J Food Drug Anal. 2022 Mar 15;30(1):26-37. doi: 10.38212/2224-6614.3399.
Despite the fact that carbamates and organophosphates cause acute poisoning via different mechanisms and require disparate management, they are indistinguishable by current clinical assays. Herein, direct immersion solid-phase microextraction (DI-SPME) plus thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was developed to discern them. Both pesticides spiked in human serum were extracted by SPME and analyzed by TD-ESI/MS/MS. This is a promising emergency care platform as rapid analyses could be done in tiny sample volumes with satisfactory recovery (89.46%-116.32%), precision (covariance <20%), sensitivity (LOD <0.1 μg/mL), turnaround time (<5 minutes), and linearity (R = 0.9827-0.9992) within 0.1-100 μg/mL.
尽管氨基甲酸酯类和有机磷类通过不同的机制引起急性中毒，需要不同的处理方法，但目前的临床检测方法无法将它们区分开来。在此，我们开发了直接浸入固相微萃取（DI-SPME）和热解吸-电喷雾串联质谱（TD-ESI/MS/MS）来区分它们。两种农药均以人血清为基质，通过 SPME 提取并通过 TD-ESI/MS/MS 进行分析。这是一种很有前途的急救护理平台，因为它可以在小体积样品中进行快速分析，具有令人满意的回收率（89.46%-116.32%）、精密度（方差<20%）、灵敏度（LOD<0.1μg/mL）、分析时间（<5 分钟）和线性范围（0.1-100μg/mL 范围内 R = 0.9827-0.9992）。
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