血清 25-羟维生素 D 与子宫内膜 HOXA10mRNA 的表达相关。

Serum 25-hydroxyvitamin D correlates with endometrial HOXA10 mRNA expression.

机构信息

Department of Obstetrics and Gynecology, Altınbas University, Istanbul, Turkey.

出版信息

Eur Rev Med Pharmacol Sci. 2022 May;26(10):3483-3486. doi: 10.26355/eurrev_202205_28842.

DOI:10.26355/eurrev_202205_28842

PMID:35647828

Abstract

OBJECTIVE

Endocrine Society classified patients with serum 25-hydroxyvitamin D [25(OH)D] levels below 20 ng/ml as deficiency, 20-30 ng/ml as insufficiency, and >30 ng/ml as replete. This study was planned to investigate the relationship between serum vitamin D level and homeobox 10 mRNA expression in women with polycystic ovary syndrome (PCOS).

PATIENTS AND METHODS

Thirty women with PCOS who failed the first IVF/ICSI attempt and were decided to have endometrial injury before second attempt were included in the study. Before the endometrial injury, the serum vitamin D levels of the women were measured, and they were divided into three equal groups as proposed by the Endocrine Society. Group 1 consisted of vitamin D deficient women (<20 ng/mL), Group 2 consisted of vitamin D insufficient women (20-30 ng/mL), and Group 3 consisted of vitamin D replete women (>30 ng/mL). Women in each group were injured with a Pipelle cannula during mid-luteal phase. Endometrial samples collected during injury were analyzed for HOXA10 mRNA expression by RT-PCR and correlated with serum vitamin D level.

RESULTS

When analyzing the results according to different vitamin D thresholds, as proposed by the Endocrine Society, HOXA10 mRNA expression was comparable between vitamin D deficient and vitamin D insufficient women. The HOXA10 mRNA expression of vitamin D replete women(Group 3) was found to be higher than both vitamin D deficient (Group 1) and vitamin D insufficient women (Group 2). HOXA10 mRNA expression of the women in Group 3 was 3.3-fold higher than Group 1 and 2.6-fold higher than Group 2. HOXA10 mRNA expression was correlated to the levels of vitamin D in the Group 3 (r=0.655, p=0.02). There was no significant correlation between serum vitamin D levels and endometrial HOXA10 mRNA expression of women in both Group 1 (r=0.343, p=0.06) and Group 2 (r=0.456, p=0.08).

CONCLUSIONS

Endometrium of women with PCOS with sufficient serum vitamin D levels express significantly higher HOXA10 mRNA than patients with low serum vitamin D levels.

摘要

目的

内分泌学会将血清 25-羟维生素 D [25(OH)D]水平低于 20ng/ml 的患者定义为缺乏，20-30ng/ml 为不足，>30ng/ml 为充足。本研究旨在探讨多囊卵巢综合征（PCOS）患者血清维生素 D 水平与同源盒 10mRNA 表达的关系。

患者和方法

30 名首次体外受精/卵胞浆内单精子注射（IVF/ICSI）失败的 PCOS 患者被纳入本研究，这些患者决定在第二次尝试前进行子宫内膜损伤。在进行子宫内膜损伤前，测量了这些女性的血清维生素 D 水平，并根据内分泌学会的建议将她们分为三组。第 1 组为维生素 D 缺乏组（<20ng/ml），第 2 组为维生素 D 不足组（20-30ng/ml），第 3 组为维生素 D 充足组（>30ng/ml）。每组女性在黄体中期均使用 Pipelle 管进行损伤。通过 RT-PCR 分析损伤时采集的子宫内膜样本中的 HOXA10mRNA 表达，并与血清维生素 D 水平相关。

结果

根据内分泌学会提出的不同维生素 D 阈值分析结果，HOXA10mRNA 表达在维生素 D 缺乏和维生素 D 不足的女性之间无差异。维生素 D 充足组（第 3 组）的 HOXA10mRNA 表达高于维生素 D 缺乏组（第 1 组）和维生素 D 不足组（第 2 组）。第 3 组的 HOXA10mRNA 表达水平比第 1 组和第 2 组分别高 3.3 倍和 2.6 倍。第 3 组的 HOXA10mRNA 表达与维生素 D 水平呈正相关（r=0.655，p=0.02）。第 1 组（r=0.343，p=0.06）和第 2 组（r=0.456，p=0.08）女性的血清维生素 D 水平与子宫内膜 HOXA10mRNA 表达无显著相关性。