Department of Ophtalmology, Faculty of Medical Science, Niğde Ömer Halisdemir University, Niğde, Turkey.
Department of Anatomy, Faculty of Medical Science, Niğde Ömer Halisdemir University, Niğde, Turkey.
Indian J Ophthalmol. 2022 Jun;70(6):1971-1974. doi: 10.4103/ijo.IJO_2588_21.
In this study, our aim was to investigate if fibrin adhesives used in conjunctival wound surgery with autologous conjunctival grafts could be used repeatedly at different times after surgical opening.
40 New Zealand rabbits were used in the study. These animals were divided into four groups, each consisting of 10 rabbits, and hence 20 eyes. In the first group (control group), Tisseel fibrin sealant was used on the day the eye was first opened surgically; in the second group, it was used on the seventh day; on the third group, it was used on the 14 day; and in the fourth group, it was used 28 days after surgical opening. The graft from the inferior bulbar conjunctiva was attached using Tisseel fibrin glue to the superior scleral bed at the location where the superior bulbar conjunctiva was excised in the same eye.
No microbial growth was detected in the cultures of the samples tested. There were two partial graft loss in group 2 and there was one partial graft loss in each group of the other groups, and further total graft loss was present in one rabbit in group 3. None of the rabbits had any complications like granuloma, Corneal dellen or infection.
Based on these results, fibrin sealants can be used repeatedly by storing them at room temperature. Repeated use of fibrin adhesives will reduce the cost of ophthalmologic surgeries and non-ophthalmologic surgeries.
本研究旨在探讨在自体结膜移植物行结膜伤口手术时使用纤维蛋白黏合剂，在手术切开后不同时间点重复使用是否可行。
本研究使用了 40 只新西兰兔。这些动物被分为四组，每组 10 只兔子，即每组 20 只眼睛。在第一组（对照组）中，在手术首次切开眼球当天使用 Tisseel 纤维蛋白密封剂；在第二组中，在第 7 天使用；在第三组中，在第 14 天使用；在第四组中，在手术切开 28 天后使用。从下穹窿结膜取下的移植物，使用 Tisseel 纤维蛋白胶，粘贴到同一眼切除上穹窿结膜的上方巩膜床。
未在测试样本的培养物中检测到微生物生长。在第 2 组中有两例部分移植物丢失，在其他三组中每组各有一例部分移植物丢失，在第 3 组中有一只兔子进一步发生完全移植物丢失。所有兔子均无肉芽肿、角膜失弹或感染等并发症。
基于这些结果，纤维蛋白密封剂可在室温下储存并重复使用。重复使用纤维蛋白黏合剂将降低眼科手术和非眼科手术的成本。
