Serizawa Takeshi, Maeda Tohru, Sawada Toshiki
Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1-H121 Ookayama, Meguro-ku, Tokyo 152-8550, Japan.
Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi-shi, Saitama 332-0012, Japan.
ACS Macro Lett. 2020 Mar 17;9(3):301-305. doi: 10.1021/acsmacrolett.9b01008. Epub 2020 Feb 12.
Molecular self-assembly in solutions is a powerful strategy for fabricating functional architectures. Various bio(macro)molecules have been used as self-assembly components. However, structural polysaccharides, such as cellulose and chitin, have rarely been a research focus for molecular self-assembly, even though their crystalline assemblies potentially have robust physicochemical properties. Herein, we demonstrated the neutralization-induced self-assembly of cellulose oligomers into antibiofouling crystalline nanoribbon networks to produce physically cross-linked hydrogels. The self-assembly proceeded even in versatile complex mixtures, such as serum-containing cell culture media, in a controlled manner for 3D cell culture. The cultured cells grew into cell aggregates (spheroids), which were simply collected through natural filtration due to the mechanically crushable property of the crystalline nanoribbons through water flow by pipetting. We will show the potential of cellulose oligomers for biocompatible, crystalline soft materials.
溶液中的分子自组装是构建功能结构的一种强大策略。各种生物（宏观）分子已被用作自组装组件。然而，结构多糖，如纤维素和几丁质，很少成为分子自组装的研究重点，尽管它们的晶体组装体可能具有强大的物理化学性质。在此，我们展示了纤维素寡聚物在中和作用下自组装成抗生物污损的晶体纳米带网络，以产生物理交联水凝胶。即使在通用的复杂混合物中，如含血清的细胞培养基中，自组装也能以可控的方式进行，用于三维细胞培养。培养的细胞生长成细胞聚集体（球体），由于晶体纳米带通过移液管水流具有机械可压碎性，通过自然过滤即可简单收集。我们将展示纤维素寡聚物在生物相容性晶体软材料方面的潜力。
