Neutralization-Induced Self-Assembly of Cellulose Oligomers into Antibiofouling Crystalline Nanoribbon Networks in Complex Mixtures.

Molecular self-assembly in solutions is a powerful strategy for fabricating functional architectures. Various bio(macro)molecules have been used as self-assembly components. However, structural polysaccharides, such as cellulose and chitin, have rarely been a research focus for molecular self-assembly, even though their crystalline assemblies potentially have robust physicochemical properties. Herein, we demonstrated the neutralization-induced self-assembly of cellulose oligomers into antibiofouling crystalline nanoribbon networks to produce physically cross-linked hydrogels. The self-assembly proceeded even in versatile complex mixtures, such as serum-containing cell culture media, in a controlled manner for 3D cell culture. The cultured cells grew into cell aggregates (spheroids), which were simply collected through natural filtration due to the mechanically crushable property of the crystalline nanoribbons through water flow by pipetting. We will show the potential of cellulose oligomers for biocompatible, crystalline soft materials.