Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia.
School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA 5005, Australia.
J Agric Food Chem. 2022 Jun 15;70(23):7139-7147. doi: 10.1021/acs.jafc.2c02298. Epub 2022 Jun 1.
Enzymes are important catalysts for biological processes due to their high catalytic activity and selectivity. However, their low thermal stability limited their industrial applications. The present work demonstrates a simple and effective method for enzyme immobilization via spray drying. Alginate was used as a support material. Phytase, an important enzyme in the animal feed industry, was selected to study the effect of enzyme immobilization using alginate particles on its thermal stability. The physicochemical properties of alginate particles such as size, surface morphology, and heat resistance were studied. Successful immobilization of phytase was confirmed by confocal microscopy, and the immobilized phytase retained 58% of its original activity upon heating at 95 °C, compared to 4% when the alginate support material was absent. Phytase was released promptly in a simulated gastrointestinal tract with >95% of its original activity recovered. The spray drying method for phytase immobilization is scalable and applicable to other enzymes for various applications.
酶是生物过程中的重要催化剂，因为它们具有高催化活性和选择性。然而，它们的热稳定性低限制了它们的工业应用。本工作展示了一种通过喷雾干燥进行酶固定化的简单有效方法。海藻酸钠被用作支持材料。植酸酶是动物饲料工业中的一种重要酶，被选择来研究使用海藻酸钠颗粒固定化酶对其热稳定性的影响。研究了海藻酸钠颗粒的物理化学性质，如粒径、表面形貌和耐热性。通过共聚焦显微镜证实了植酸酶的成功固定化，固定化植酸酶在 95°C 加热时保留了其原始活性的 58%，而没有海藻酸钠载体时仅保留了 4%。植酸酶在模拟胃肠道中迅速释放，超过 95%的原始活性得到恢复。喷雾干燥法用于植酸酶固定化是可扩展的，适用于其他酶在各种应用中的应用。
