Salamah A A, Charnetzky W T
Acta Microbiol Hung. 1986;33(3):193-201.
The loss of the 47 megadalton (Mdal) plasmid of Yersinia pestis resulted in increased gentian violet uptake and increased sensitivity to novobiocin. The addition of calcium caused a decrease in gentian violet uptake and a decrease in novobiocin sensitivity independent of the plasmid. Differentiation of cells bearing and lacking the plasmid was accomplished using novobiocin in the presence of calcium at 26 degrees C. The novobiocin resistance levels of cells lacking the 47 Mdal plasmid and cells with the plasmid, but with an insertion in or a deletion in, or encomposing, the calcium dependence region of this plasmid, indicated that the resistance to novobiocin was not associated with the calcium dependence region, but was associated with a separate 47 Mdal plasmid region. Examination of Yersinia pseudotuberculosis and Yersinia enterocolitica isolates demonstrated that functionally similar sites are present on the plasmids that encode for calcium dependence in these species.
鼠疫耶尔森菌47兆道尔顿（Mdal）质粒的缺失导致结晶紫摄取增加和对新生霉素的敏感性增加。添加钙会导致结晶紫摄取减少以及与质粒无关的新生霉素敏感性降低。在26℃下于含钙条件下使用新生霉素，可实现携带和缺乏该质粒的细胞的区分。缺乏47 Mdal质粒的细胞以及带有该质粒但在该质粒的钙依赖性区域存在插入、缺失或包含该区域的细胞的新生霉素抗性水平表明，对新生霉素的抗性与钙依赖性区域无关，而是与一个单独的47 Mdal质粒区域有关。对假结核耶尔森菌和小肠结肠炎耶尔森菌分离株的检测表明，在这些物种中编码钙依赖性的质粒上存在功能相似的位点。
