Zarenkov M I, Goncharov E G
Mol Gen Mikrobiol Virusol. 1989 Dec(12):17-21.
The strains of Yersinia pestis that restrict their growth on the media deficient for Mg2+ ions at 37 degrees C have been found. The bacterial cell lysis is registered under these conditions. The effect of Yersinia pestis own plasmids on the level of growth restriction in the absence of Mg2+ ions has been studied. The phenomenon is not connected with the presence of the plasmid determining Ca2(+)-dependence. The presence of 6Md plasmid coding for pesticinogenicity increased the frequency of colony formation, while the heavy plasmid determining the production of "mouse" toxin favoured the increase in growth restriction on Mg2(+)-less media. The clones growing under the latter conditions acquire the rearrangements in the DNA of the plasmid coding for the "mouse" toxin.
已发现鼠疫耶尔森菌的一些菌株在37℃时在缺乏Mg2+离子的培养基上生长受限。在这些条件下可观察到细菌细胞裂解。研究了鼠疫耶尔森菌自身质粒对在无Mg2+离子时生长受限水平的影响。该现象与决定Ca2+依赖性的质粒的存在无关。编码杀鼠菌素的6Md质粒的存在增加了菌落形成频率,而决定“鼠”毒素产生的重质粒则有利于在无Mg2+培养基上生长受限程度的增加。在后者条件下生长的克隆在编码“鼠”毒素的质粒DNA中发生了重排。