College of Chemistry and Chemical Engineering, Yantai University, 30 Qingquan Road, Yantai 264005, China.
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao 266042, China.
Anal Chem. 2022 Jun 14;94(23):8317-8326. doi: 10.1021/acs.analchem.2c00800. Epub 2022 Jun 1.
Development of a flexible, easy-to-use, and well-controllable DNA-based sensing platform would provide enormous opportunities to boost molecular diagnosis and signal transduction or information processing. Herein, a duplex DNA scaffold containing a bulge was deployed for the fabrication of a simple and general DNA-based electrochemical sensing platform. It could be harnessed for different signal output behaviors (one signal-off and two signal-on modes) toward a single-step analysis of the target protein. The detection limit toward the target protein could reach about 0.1 nM. Also, it could be used as a streamlined electrochemical workflow for the successive monitoring of protein binding. Furthermore, such an electrochemical sensing platform could be explored for the operation of the concatenated AND logic gates as a molecular keypad lock system. The current sensing platform based on only one duplex DNA scaffold presented features such as simple biosensor design and fabrication, flexible operation for different signal outputs, sensitive and selective protein detection, and expandable logic operation. It thus would pave a broad road toward the development of high-performance biosensors or logic devices to be applied for molecular diagnosis or computing.
开发一种灵活、易用且易于控制的基于 DNA 的传感平台将为分子诊断和信号转导或信息处理提供巨大的机会。本文中，设计了一种含有凸起结构的双链 DNA 支架，用于构建简单且通用的基于 DNA 的电化学生物传感平台。该平台可用于不同的信号输出行为（信号关闭和信号开启两种模式），实现对目标蛋白的一步分析。该方法对目标蛋白的检测限可达到约 0.1 nM。此外，该平台还可用于连续监测蛋白结合的简化电化学生物流程。此外，这种电化学生物传感平台可作为分子键盘锁系统用于级联 AND 逻辑门的操作。该基于单双链 DNA 支架的电流传感平台具有设计和制造简单的生物传感器、灵活的多种信号输出操作、灵敏和选择性的蛋白检测以及可扩展的逻辑操作等特点。因此，该平台将为高性能生物传感器或逻辑器件的开发铺平道路，以应用于分子诊断或计算。
