绒膜来源的围产期间质干细胞改善心功能和血管再生：缺血性心脏病的优先治疗方法。

Chorion-derived perinatal mesenchymal stem cells improve cardiac function and vascular regeneration: Preferential treatment for ischemic heart disease.

机构信息

Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea; Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.

Department of Medical Life Science, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea; Division of Cardiology, Department of Internal Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul 06591, Korea.

出版信息

Hellenic J Cardiol. 2022 Jul-Aug;66:52-58. doi: 10.1016/j.hjc.2022.05.010. Epub 2022 May 29.

DOI:10.1016/j.hjc.2022.05.010

PMID:35649476

Abstract

BACKGROUND

Stem cell therapy has emerged as a novel treatment for heart failure after myocardial infarction (Ml). Bone marrow-derived mesenchymal stem cells (BM-MSCs) are commonly considered because of their accessibility and usability. However, their therapeutic potential remains controversial. In our previous in vitro study, chorion-derived mesenchymal stem cells (C-MSCs) and umbilical cord-derived mesenchymal stem cells (UC-MSCs) demonstrated an ability to differentiate into cardiomyocytes and neural cells, respectively. Thus, we examined whether C-MSCs had a better differentiation potential in an MI animal model.

METHODS

MI was induced by ligation of the left anterior descending artery, and DiI-labeled MSCs were injected into the border of the infarcted myocardium. The left ventricular ejection fraction (LVEF) and fractional shortening (FS) were measured using echocardiograms. Masson's Trichrome staining was performed to evaluate the viable myocardium. Alpha-sarcomeric actin (α-SA), cardiac troponin-T (cTnT), and isolectin were immunolabeled to evaluate differentiation and capillary formation.

RESULTS

After 8 weeks, the LVEF and FS significantly increased to a greater extent in the C-MSC-injected group with maintenance of viable myocardium, as compared to in the control, UC-MSC-, and BM-MSC-injected groups (p < 0.05). Compared to UC-MSCs and BM-MSCs, C-MSCs significantly increased the capillary density (p < 0.05) and demonstrated higher expressions of cTnT and α-SA.

CONCLUSIONS

In conclusion, compared to UC-MSCs and BM-MSCs, C-MSCs showed a better therapeutic efficacy in a rat MI model.

摘要

背景

干细胞疗法已成为心肌梗死后心力衰竭的一种新的治疗方法。由于其可及性和可用性，骨髓间充质干细胞（BM-MSCs）通常被认为是可行的选择。然而，它们的治疗潜力仍存在争议。在我们之前的体外研究中，绒毛膜来源的间充质干细胞（C-MSCs）和脐带来源的间充质干细胞（UC-MSCs）分别显示出分化为心肌细胞和神经细胞的能力。因此，我们研究了 C-MSCs 在心肌梗死动物模型中是否具有更好的分化潜力。

方法

通过结扎左前降支诱导心肌梗死，将 DiI 标记的 MSC 注射到梗死心肌的边缘。使用超声心动图测量左心室射血分数（LVEF）和缩短分数（FS）。通过 Masson 三色染色评估存活心肌。免疫标记α-横纹肌肌动蛋白（α-SA）、心肌肌钙蛋白-T（cTnT）和异硫氰酸荧光素评估分化和毛细血管形成。

结果

8 周后，与对照组、UC-MSC 组和 BM-MSC 组相比，C-MSC 组的 LVEF 和 FS 显著增加，且存活心肌得以维持（p < 0.05）。与 UC-MSCs 和 BM-MSCs 相比，C-MSCs 显著增加了毛细血管密度（p < 0.05），并表现出更高的 cTnT 和 α-SA 表达。