School of Chemistry and Environmental Engineering, Sichuan University of Science & Engineering, Sichuan, Zigong, 643000, China.
School of Materials Science and Engineering, Tianjin University of Technology, Tianjin, Xiqing District, 300384, China.
Anal Chim Acta. 2022 Jun 29;1214:339939. doi: 10.1016/j.aca.2022.339939. Epub 2022 May 14.
In current work, one novel benzothiazole-based fluorescent chemosensor (TZ-BO) with ESIPT luminescence mechanism was designed for the detection of hydrogen peroxide (HO) as typical reactive oxygen species (ROS). The boronate catenary in our designed probe molecule TZ-BO is both the blocking group of ESIPT and the reactive group of HO. The synthesized probe TZ-BO has the advantages of large Stokes shift, high sensitivity, good selectivity, and fast reaction speed to HO. The ability of the probe to detect HO in aqueous media and exogenous HO in cells by fluorescence signal was investigated. The experimental results manifested that probe TZ-BO is able to sensitively detect HO using a fluorescence method in an excellent linear range of HO concentration from 0 to 60 μM (R = 0.994). The detection limit was determined as 1.0 × 10 M. Most importantly, the probe TZ-BO with highly low cytotoxicity can effectively detect exogenous HO in HeLa cells. This probe provides a promising tool for monitoring the real concentration level of HO in related physiological and pathological researches.
在当前的工作中，设计了一种基于苯并噻唑的新型荧光化学传感器（TZ-BO），具有 ESIPT 发光机制，用于检测过氧化氢（HO）作为典型的活性氧（ROS）。我们设计的探针分子 TZ-BO 中的硼酸酯链既是 ESIPT 的封锁基团，也是 HO 的反应基团。合成的探针 TZ-BO 具有大斯托克斯位移、高灵敏度、良好选择性和快速反应速度等优点，可用于检测水相介质和细胞中外源性 HO 的荧光信号。实验结果表明，探针 TZ-BO 能够通过荧光法灵敏地检测 HO，HO 浓度在 0 到 60 μM 的范围内呈良好的线性关系（R = 0.994）。检测限确定为 1.0×10 -6 M。最重要的是，具有低细胞毒性的探针 TZ-BO 可以有效地检测 HeLa 细胞中的外源性 HO。该探针为监测相关生理和病理研究中 HO 的实际浓度水平提供了一种有前途的工具。
