Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050, Brussel, Belgium.
Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050, Brussel, Belgium.
Anal Chim Acta. 2022 Jun 29;1214:339955. doi: 10.1016/j.aca.2022.339955. Epub 2022 May 23.
In this contribution, we review the recent literature relating to the measurement and modelling of all diffusion-dominated processes contributing to the efficiency of a chromatographic column. In first instance, this involves the measurement and modelling of the overall effective diffusion coefficient D (determining the so-called B-term band broadening). The latter manifests itself most clearly during a so-called peak parking experiment. Using effective medium theory modelling, the measured D-value can subsequently be decomposed into its constituent contributions, of which the intra-particle or the mesoporous zone and the surface diffusion coefficient are the most important ones. As an accurate estimation of the diffusion processes also allows computing the C-term plate height contribution terms, the review ends with some recent insights obtained when using the established B- and C-term contributions to compute the degree of eddy-dispersion in contemporary packed bed columns.
在本贡献中，我们回顾了与测量和建模所有扩散主导过程相关的最新文献，这些过程有助于色谱柱的效率。首先，这涉及到整体有效扩散系数 D 的测量和建模（确定所谓的 B 项带展宽）。在所谓的峰停实验中，后者表现得最为明显。使用有效介质理论模型，随后可以将测量的 D 值分解为其组成贡献，其中最重要的是颗粒内或中孔区和表面扩散系数。由于对扩散过程的准确估计还允许计算 C 项板高贡献项，因此当使用已建立的 B 和 C 项贡献来计算现代填充床色谱柱中的涡流扩散程度时，我们在审查结束时获得了一些最新的见解。
