揭示天然马铃薯淀粉与矢车菊素-3-O-葡萄糖苷之间 pH 调控相互作用的新见解：静电相互作用依赖性结合。
Insights into pH-modulated interactions between native potato starch and cyanidin-3-O-glucoside: Electrostatic interaction-dependent binding.
机构信息
College of Food Science and Nutritional Engineering, China Agricultural University, National Engineering Research Centre for Fruit and Vegetable Processing, Key Lab of Fruit and Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory for Food Nonthermal Processing, Beijing 100083, China.
College of Food Science and Nutritional Engineering, China Agricultural University, National Engineering Research Centre for Fruit and Vegetable Processing, Key Lab of Fruit and Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory for Food Nonthermal Processing, Beijing 100083, China.
出版信息
Food Res Int. 2022 Jun;156:111129. doi: 10.1016/j.foodres.2022.111129. Epub 2022 Mar 14.
Potato starch (PS), a natural and structured semicrystalline polymer, exhibits a distinct capacity to bind cyanidin-3-O-glucoside (C3G) at different pH values (3, 5, and 7). In this study, levels of NaCl (0.05%, 0.5%, and 5%, w/v) were introduced as electrostatic disturbing agents to the PS-C3G complexes to quantitatively reveal the role electrostatic interactions played in the binding at different pH conditions. The binding rate dropped from 31.60% to 2.19% as the pH value shifted from 3 to 7, indicating a decreasing affinity from C3G to PS. Further zeta potentials showed the possibility of electrostatic interactions in the PS-C3G complexes at pH 3 as well as screened charges in the presence of NaCl. The binding rate at pH 3 then exhibited a progressive decline to a final approximate one-third as the concentration of NaCl increased, which implied that the electrostatic interactions constituted the primary two-thirds of the driving forces involving complex stability at pH 3. The results of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, however, showed that hydrogen bonds had a negligible effect on the binding of C3G to PS at all pH conditions. Finally, the confocal laser scanning microscopy (CLSM) images, particle size and color differences were consistent with the change in the binding rate. Overall, electrostatic interactions were considered the key pH-modulated interactions between PS and C3G at different pH conditions.
马铃薯淀粉（PS）是一种天然的、结构半结晶的聚合物，在不同的 pH 值（3、5 和 7）下，对矢车菊素-3-O-葡萄糖苷（C3G）表现出明显的结合能力。在这项研究中，引入不同浓度的 NaCl（0.05%、0.5%和 5%，w/v）作为静电干扰剂，用于研究 PS-C3G 复合物，以定量揭示静电相互作用在不同 pH 条件下结合中的作用。当 pH 值从 3 变为 7 时，结合率从 31.60%下降到 2.19%，表明 C3G 与 PS 的亲和力降低。进一步的 Zeta 电位表明，在 pH 3 时，PS-C3G 复合物中存在静电相互作用的可能性以及在 NaCl 存在下筛选的电荷。当 NaCl 浓度增加时，pH 3 时的结合率逐渐下降到最终的约三分之一，这意味着静电相互作用构成了复合物稳定性的主要三分之二驱动力，而 pH 3 时的驱动力。衰减全反射傅里叶变换红外光谱（ATR-FTIR）的结果表明，在所有 pH 条件下，氢键对 C3G 与 PS 的结合几乎没有影响。最后，共焦激光扫描显微镜（CLSM）图像、粒径和颜色差异与结合率的变化一致。总的来说，静电相互作用被认为是 PS 和 C3G 在不同 pH 值下的关键 pH 调节相互作用。
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