Laboratório de Química de Produtos Naturais, Programa de Pós graduação de Biotecnologia Aplicada a Agricultura, Universidade Paranaense, Umuarama Paraná, Brasil.
Laboratório de Microbiologia, Programa de Pós graduação de Biotecnologia Aplicada a Agricultura, Universidade Paranaense, Umuarama Paraná, Brasil.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2022 Jul;39(7):1296-1310. doi: 10.1080/19440049.2022.2080870. Epub 2022 Jun 2.
is known for its richness in essential oil which has been widely investigated due to its biological activities such as antimicrobial, insecticidal, trypanocidal, antimalarial and antioxidant. The objective of this work was to chemically analyze and evaluate the antifungal and antimycotoxigenic activity of the essential oil and the crude extract of leaves, flower buds and stems of from the northwest region of the state of Paraná. The essential oil was obtained by hydrodistillation using a Clevenger-type apparatus. To obtain the crude extract, the leaves, flower buds and stems were pulverized and subjected to a dynamic maceration process using 70% v v ethyl alcohol. Chemical analysis of the essential oil was performed by GC/MS, and chemical identification of the crude extract by UHPLC-ESI/qTOF. Antifungal activity (, , , and ) was performed by broth microdilution and the antimycotoxigenic assay was performed with and . Ochratoxin A was extracted by partition with chloroform and quantified by HPLC-FL. The oil yield was 0.29% for leaves, 0.34% for stems and 0.38% for flower buds, and the major compounds were fenchone, β-caryophyllene, α-cadinol, 14-hydroxy-9- epi-caryophyllene, 9β,13β-epoxy-7-abietene, α-cadinol and 6-7-dehydroroyleanone. The main chemical compounds identified in the crude extract were terpenes, anthocyanins, flavonoids, tannins and phenolic acids. The minimum inhibitory concentration (MIC) of oils from leaves, flower buds and stems for the strains tested ranged from 0.87mg mL to 33.3 mg mL, while the minimum fungicidal concentration (MFC) ranged from 6.94 mg mL and 33.3 mg mL. The MIC and MFC for ketoconazole, tebuconazole, sorbate and nitrite ranged from 0.05 to 33.3 mg mL. The oil and crude extract of leaves, stems and flower buds showed an inhibition of ochratoxin A production for of approximately 100%.
巴西樟从西北巴拉那州的叶子、花蕾和茎中提取的精油和粗提取物具有丰富的精油，由于其具有抗菌、杀虫、杀锥虫、抗疟和抗氧化等生物活性，因此被广泛研究。本工作的目的是对巴西樟叶子、花蕾和茎的精油和粗提取物进行化学分析和评估其抗真菌和抗真菌毒素活性。使用克利夫兰型装置通过水蒸馏获得精油。为了获得粗提取物，将叶子、花蕾和茎粉碎，并通过动态浸提过程用 70%v/v 乙醇处理。通过 GC/MS 对精油进行化学分析，通过 UHPLC-ESI/qTOF 对粗提取物进行化学鉴定。通过肉汤微量稀释法进行抗真菌活性（、、、和），并用和进行抗真菌毒素测定。用氯仿萃取 ochratoxin A，并用 HPLC-FL 定量。叶、茎和花蕾的油产量分别为 0.29%、0.34%和 0.38%，主要化合物为葑酮、β-石竹烯、α-卡杜醇、14-羟基-9-表-石竹烯、9β，13β-环氧-7-贝壳烯、α-卡杜醇和 6-7-脱水罗伊醇酮。粗提取物中的主要化学化合物为萜类化合物、花青素、类黄酮、单宁和酚酸。测试菌株的叶、花蕾和茎的油的最小抑菌浓度（MIC）范围为 0.87mg/mL 至 33.3mg/mL，而最小杀菌浓度（MFC）范围为 6.94mg/mL 至 33.3mg/mL。酮康唑、噻菌灵、山梨酸钾和亚硝酸盐的 MIC 和 MFC 范围为 0.05 至 33.3mg/mL。叶、茎和花蕾的油和粗提取物对 ochratoxin A 的产生抑制约为 100%。
