将L-天冬酰胺酶封装到不对称可渗透聚合物囊泡中。

l-Asparaginase Encapsulation into Asymmetric Permeable Polymersomes.

作者信息

Bueno Cecilia Z, Apolinário Alexsandra C, Duro-Castano Aroa, Poma Alessandro, Pessoa Adalberto, Rangel-Yagui Carlota O, Battaglia Giuseppe

机构信息

Department of Biochemical and Pharmaceutical Technology, University of São Paulo, 05508-000 São Paulo, Brazil.

Department of Chemistry, University College London, WC1H 0AJ London, United Kingdom.

出版信息

ACS Macro Lett. 2020 Oct 20;9(10):1471-1477. doi: 10.1021/acsmacrolett.0c00619. Epub 2020 Oct 9.

DOI:10.1021/acsmacrolett.0c00619

PMID:35653665

Abstract

This work reports, for the encapsulation of l-asparaginase, an anticancer enzyme into hybrid PMPC-PDPA/PEO-PBO asymmetric polymersomes previously developed by our group, with loading capacities with over 800 molecules per vesicle. Enzyme-loaded polymersomes show permeability and capacity to hydrolyze l-asparagine, which is essential to cancer cells. The nanoreactors proposed in this work can be potentially used in further studies to develop novel therapeutic alternatives based on l-asparaginase.

摘要

本研究报道了将抗癌酶L-天冬酰胺酶封装到我们小组之前开发的杂化聚甲基丙烯酸2-羟乙酯-聚多巴胺/聚环氧乙烷-聚硼酸酯不对称聚合物囊泡中的情况，其负载量超过每个囊泡800个分子。载酶聚合物囊泡表现出对L-天冬酰胺的渗透性和水解能力，而L-天冬酰胺对癌细胞至关重要。本研究中提出的纳米反应器可能会在进一步的研究中用于开发基于L-天冬酰胺酶的新型治疗方案。