链脲佐菌素诱导糖尿病大鼠膈神经复合肌肉动作电位的变化。

Changes in phrenic nerve compound muscle action potential in streptozotocin-induced diabetic rats.

机构信息

Laboratory of Cardiovascular Physiology and Pathophysiology, Department of Physiology, Federal University of Paraná, Brazil.

出版信息

Respir Physiol Neurobiol. 2022 Sep;303:103923. doi: 10.1016/j.resp.2022.103923. Epub 2022 May 30.

DOI:10.1016/j.resp.2022.103923

PMID:35654367

Abstract

PURPOSE

To evaluate the phrenic nerve compound muscle action potential (CMAP) in rats after diabetes mellitus (DM) induction.

METHODS

Twenty DM animals (intravenous streptozotocin, 45 mg.kg) and 25 controls underwent CMAP analysis before and 30, 60 and 90 days after DM induction.

RESULTS

Amplitude (mV) progressively declined in DM group after 30 (Mean difference (MD): -0.915, 95 % Confidence interval (CI) -1.580 to -0.250, p < 0.01), 60 (MD: -1.122, 95 % CI -1.664 to -0.581, p < 0.001) and 90 days (MD: -2.226, 95 % CI -3.059 to -1.393, p < 0.001); as well as the area (mV.ms) after 30 (MD: -3.19, 95 % CI -5.94 to -0.44, p < 0.05), 60 (MD: -3.94, 95 % CI -6.24 to -1.64, p < 0.001) and 90 days (MD: -8.64, 95 % CI -12.08 to -5.21, p < 0.001). Transient differences were observed in latency and duration at 60 days.

CONCLUSIONS

The progressive changes in phrenic nerve CMAP observed during DM suggest a decrement in axonal function rather than substantial demyelination.

摘要

目的

评估糖尿病（DM）诱导后大鼠膈神经复合肌肉动作电位（CMAP）。

方法

20 只 DM 动物（静脉注射链脲佐菌素，45mg/kg）和 25 只对照动物在 DM 诱导前以及 30、60 和 90 天后进行 CMAP 分析。

结果

DM 组在 30 天后（平均差值（MD）：-0.915，95%置信区间（CI）：-1.580 至 -0.250，p<0.01）、60 天后（MD：-1.122，95%CI：-1.664 至 -0.581，p<0.001）和 90 天后（MD：-2.226，95%CI：-3.059 至 -1.393，p<0.001），振幅（mV）逐渐下降；30 天后（MD：-3.19，95%CI：-5.94 至 -0.44，p<0.05）、60 天后（MD：-3.94，95%CI：-6.24 至 -1.64，p<0.001）和 90 天后（MD：-8.64，95%CI：-12.08 至 -5.21，p<0.001），面积（mV.ms）也逐渐下降。潜伏期和持续时间在 60 天时有短暂差异。