Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Biomed Res Int. 2022 May 12;2022:2028082. doi: 10.1155/2022/2028082. eCollection 2022.
Cervical cancer is rated to be the leading cause of cancer-related death in women worldwide. Since screening test and conventional treatments are less accessible for people in developing countries, an alternative use of medicinal plants exhibiting strong anticancer activities may be an affordable means to treat cervical cancer. (MC) is the newly identified species; however, its biological functions including anticancer activities have been largely unexplored. Hence, in this study, we were interested in investigating anticancer effects of this plant on the human cervical cell line (HeLa). MC extract was profiled for phytochemicals by TLC. This plant was tested to contain alkaloids, flavonoids, and terpenes. HeLa cells were treated with MC extract to investigate the anticancer activities. Cytotoxicity and viability of cells treated with MC were determined by MTT assay and Trypan blue exclusion assay. Cell migration was tested by wound healing assay, and cell invasion was determined by Transwell assay. The level of caspase 7, caspase 9, and PARP was determined by western blot analysis. We found that the leaf extract of MC strongly reduced cancer cell survival rate. This finding was consistent with the discovery that the extract dramatically induced apoptosis of cervical cancer cells through the activation of caspase 7 and caspase 9 which consequently degraded PARP protein. Furthermore, MC extract at lower concentrations which were not cytotoxic to the cancer cells showed potent inhibitory activities against HeLa cervical cancer cell migration and invasion. possesses its pharmacological properties in inhibiting cervical cancer cell migration/invasion and inducing apoptotic signaling. This accumulated information suggests that may be a beneficial source of potential agents for cervical cancer treatment.
宫颈癌被评为全球女性癌症相关死亡的主要原因。由于发展中国家的人们较难获得筛查测试和常规治疗，因此使用具有强烈抗癌活性的药用植物可能是一种负担得起的治疗宫颈癌的方法。(MC)是新鉴定的物种;然而，其生物功能，包括抗癌活性，在很大程度上尚未得到探索。因此，在这项研究中，我们有兴趣研究这种植物对人宫颈癌细胞系(HeLa)的抗癌作用。用 TLC 对 MC 提取物进行了植物化学分析。该植物被检测含有生物碱、类黄酮和萜类化合物。用 MC 提取物处理 HeLa 细胞，以研究其抗癌活性。用 MTT 法和台盼蓝排斥试验测定细胞经 MC 处理后的细胞毒性和活力。用划痕愈合试验检测细胞迁移，用 Transwell 试验检测细胞侵袭。用 Western blot 分析测定 caspase 7、caspase 9 和 PARP 的水平。我们发现 MC 的叶提取物强烈降低了癌细胞的存活率。这一发现与以下发现一致，即提取物通过激活 caspase 7 和 caspase 9 强烈诱导宫颈癌细胞凋亡，从而降解 PARP 蛋白。此外，在对癌细胞无细胞毒性的较低浓度下，MC 提取物对 HeLa 宫颈癌细胞迁移和侵袭具有强大的抑制活性。具有抑制宫颈癌细胞迁移/侵袭和诱导凋亡信号的药理学特性。这些累积的信息表明，可能是治疗宫颈癌的潜在药物的有益来源。
