Toktay Erdem, Tastan Tugba Bal, Gurbuz Muhammet Ali, Erbas Elif, Demir Ozlem, Ugan Rustem Anıl, Selli Jale
Kafkas University, Faculty of Medicine, Department of Histology and Embryology, Kars, Turkey.
Ataturk University, Faculty of Medicine, Department of Histology and Embryology, Erzurum, Turkey.
Iran J Basic Med Sci. 2022 Feb;25(2):173-178. doi: 10.22038/IJBMS.2022.61289.13559.
We thought that astaxanthin (ASX) might be a protective agent in oxidative stress damage that develops against ischemia and reperfusion injury in the rat ovary.
The experimental groups consisted of healthy, I (Ischemia), I+ASX50, I+ASX100, I/R (Ischemia/Reperfusion), I/R+ ASX50, and I/R+ ASX100. Vascular clamps were applied to the ovaries for 3 hr to induce ischemia. For the reperfusion groups, the clamps were opened and blood flow was restored to the ovaries for 3 hr. At the end of the experiment, biochemical, histopathological, and immunohistochemical analyses were made from the tissue samples taken.
While MDA levels increased significantly in I and I/R groups, SOD levels decreased. It was found that ASX significantly decreased MDA levels and increased SOD activity in treatment groups depending on the dose. Caspase 3, IL-1 β, and IL-6 expressions were severely increased in ischemia and I/R groups, while the severity of I+ASX50 and I/R+ASX100 immunoreactivity was decreased. While severe hemorrhage areas were observed in I and IR groups, minimal hemorrhage areas were observed in the treatment groups, especially in I/R+ASX100 groups. In addition, inflammatory cells and necrotic cells in the I/R group were not observed in I/R+ASX50 and I/R+ASX100 groups.
As a result, it was determined that ASX has a strong protective role against oxidative damage in the treatment of ovarian ischemia-reperfusion injury.
我们认为虾青素（ASX）可能是一种保护剂，可抵御大鼠卵巢缺血再灌注损伤所产生的氧化应激损伤。
实验组包括健康组、I（缺血）组、I + ASX50组、I + ASX100组、I/R（缺血/再灌注）组、I/R + ASX50组和I/R + ASX100组。对卵巢施加血管夹3小时以诱导缺血。对于再灌注组，松开血管夹，使卵巢恢复血流3小时。实验结束时，对所取组织样本进行生化、组织病理学和免疫组织化学分析。
I组和I/R组中丙二醛（MDA）水平显著升高，超氧化物歧化酶（SOD）水平降低。结果发现，ASX可使治疗组中的MDA水平显著降低，并根据剂量增加SOD活性。缺血组和I/R组中半胱天冬酶3、白细胞介素1β和白细胞介素6的表达显著增加，而I + ASX50组和I/R + ASX100组免疫反应性的严重程度降低。I组和IR组中观察到严重出血区域，而治疗组中，尤其是I/R + ASX100组中观察到最小出血区域。此外，I/R + ASX50组和I/R + ASX100组中未观察到I/R组中的炎性细胞和坏死细胞。
结果表明，ASX在治疗卵巢缺血再灌注损伤时对氧化损伤具有强大的保护作用。
