Robinson P H, Moran T H, Goldrich M, McHugh P R
Am J Physiol. 1987 Apr;252(4 Pt 1):G529-34. doi: 10.1152/ajpgi.1987.252.4.G529.
Autoradiography using 125I-labeled Bolton Hunter-CCK-33 was used to study the distribution of cholecystokinin binding sites at different stages of development in the rat upper gastrointestinal tract. Cholecystokinin (CCK) binding was present in the distal stomach, esophagus, and gastroduodenal junction in the rat fetus of gestational age of 17 days. In the 20-day fetus, specific binding was found in the gastric mucosa, antral circular muscle, and pyloric sphincter. Mucosal binding declined during postnatal development and had disappeared by day 15. Antral binding declined sharply between day 10 and day 15 and disappeared by day 50. Pyloric muscle binding was present in fetal stomach and persisted in the adult. Pancreatic CCK binding was not observed before day 10. These results suggest that CCK may have a role in the control of gastric emptying and ingestive behavior in the neonatal rat.
使用125I标记的博尔顿·亨特-CCK-33进行放射自显影，以研究大鼠上消化道不同发育阶段胆囊收缩素结合位点的分布。在妊娠17天的大鼠胎儿中，胆囊收缩素（CCK）结合存在于胃远端、食管和胃十二指肠交界处。在20天的胎儿中，在胃黏膜、胃窦环形肌和幽门括约肌中发现了特异性结合。出生后发育过程中黏膜结合减少，到第15天时消失。胃窦结合在第10天至第15天之间急剧下降，到第50天时消失。幽门肌结合在胎儿胃中存在，并在成年期持续存在。在第10天之前未观察到胰腺CCK结合。这些结果表明，CCK可能在新生大鼠胃排空和摄食行为的控制中起作用。
