Xu Jing, Wang Shu-Juan, Bu Shan-Shan, Guo Xiao-Qi, Ge Hong
Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan province, China.
Iran J Basic Med Sci. 2022 Jan;25(1):68-74. doi: 10.22038/IJBMS.2021.59190.13143.
This study aimed to investigate the mechanism of the anticancer effect of theaflavin (TF) in nasopharyngeal carcinoma.
CNE2 cells were used to study the anticancer effect of TF. This study used Cell Counting Kit-8 (CCK8) assay on proliferation and used flow cytometry to detect apoptosis. The protein expression of Bcl-2, Bax, caspase 3, and caspase 9 was detected by Western blot, and autophagy-related proteins were also detected.
TF inhibited proliferation of CNE2 cells, promoted apoptosis, and up-regulated the expression of caspase 3, caspase 9, and Bax, and decreased the level of Bcl-2. Unexpectedly, TF induced autophagy rather than inhibiting autophagy through up-regulating the levels of the autophagy marker light chain 3 (LC3) and Lysosomal-associated membrane protein 1 (LAMP1) and reducing levels of the autophagosome cargo protein p62, and the effect was via the mTOR pathway. Besides, autophagy inhibitor Chloroquine (CQ) suppressed the effect of TF on Bax, Bcl-2 and activation of caspase 3 and caspase 9.
TF promoted apoptosis of nasopharyngeal carcinoma cells, the mechanism was unexpectedly involved in inducing autophagy.
本研究旨在探讨茶黄素（TF）对鼻咽癌的抗癌作用机制。
采用CNE2细胞研究TF的抗癌作用。本研究使用细胞计数试剂盒-8（CCK8）检测细胞增殖情况，并采用流式细胞术检测细胞凋亡。通过蛋白质免疫印迹法检测Bcl-2、Bax、半胱天冬酶3和半胱天冬酶9的蛋白表达，同时也检测自噬相关蛋白。
TF抑制CNE2细胞增殖，促进细胞凋亡，上调半胱天冬酶3、半胱天冬酶9和Bax的表达，降低Bcl-2水平。出乎意料的是，TF通过上调自噬标记物微管相关蛋白1轻链3（LC3）和溶酶体相关膜蛋白1（LAMP1）的水平，降低自噬体货物蛋白p62的水平，从而诱导自噬而非抑制自噬，且该作用是通过mTOR通路实现的。此外，自噬抑制剂氯喹（CQ）抑制了TF对Bax、Bcl-2以及半胱天冬酶3和半胱天冬酶9激活的影响。
TF促进鼻咽癌细胞凋亡，其机制出乎意料地涉及诱导自噬。
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