Key Laboratory of Animal Science of State Ethnic Affairs Commission, Southwest Minzu University, Chengdu 610041, China.
Animal Breeding and Genetics Key Laboratory of Sichuan Province, Animal Science Academy of Sichuan Province, Chengdu 610066, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Sep;259:109389. doi: 10.1016/j.cbpc.2022.109389. Epub 2022 Jun 1.
Inflammatory chemokine CCL5 can mediate the occurrence of inflammatory reactions and participate in various disease processes. (Ch)CCL5 gene of Jintang black goat (Capra hircus, C. hircus) was cloned. The CDS (coding sequences) was 276 bp in length and encoded 91 amino acids. The 26.5 kDa recombinant protein was expressed by Escherichia coli system and purified by Ni-Agarose. The viabilities of primary goat lung fibroblasts could be enhanced after treating with ChCCL5 protein (12.5, 25, 50 μg/mL) (P < 0.05). The expression levels of interleukin-1beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor (TNF-α), C-C motif chemokine ligand 2 (CCL2) and heat-shock proteins (Hsp70) genes were upregulated after treating with ChCCL5 protein (12.5, 25, 50 μg/mL). Besides, the viabilities and phagocytic abilities of primary mouse peritoneal macrophages could be enhanced after treating with ChCCL5 protein (12.5, 25, 50 μg/mL) (P < 0.05). The expression levels of IL-1β, IL-6, toll-like receptor 4 (TLR4), inducible nitric oxide synthase (iNOs) and TNF-α genes were upregulated after treating with ChCCL5 protein (12.5, 25, 50 μg/mL) (P < 0.05). These results indicated that goat CCL5 might play a role in the inflammatory response by regulating the inflammatory cytokines produced by lung fibroblasts and macrophages.
炎性趋化因子 CCL5 可以介导炎症反应的发生，并参与各种疾病过程。（Ch）CCL5 基因被克隆。CCDS（编码序列）长 276bp，编码 91 个氨基酸。26.5kDa 的重组蛋白通过大肠杆菌系统表达，并通过 Ni-Agarose 纯化。用 ChCCL5 蛋白（12.5、25、50μg/ml）处理后，原代山羊肺成纤维细胞的活力可增强（P<0.05）。用 ChCCL5 蛋白（12.5、25、50μg/ml）处理后，白细胞介素-1β（IL-1β）、白细胞介素 6（IL-6）、肿瘤坏死因子（TNF-α）、C-C 基序趋化因子配体 2（CCL2）和热休克蛋白（Hsp70）基因的表达水平上调。此外，用 ChCCL5 蛋白（12.5、25、50μg/ml）处理后，原代小鼠腹腔巨噬细胞的活力和吞噬能力增强（P<0.05）。用 ChCCL5 蛋白（12.5、25、50μg/ml）处理后，IL-1β、IL-6、Toll 样受体 4（TLR4）、诱导型一氧化氮合酶（iNOS）和 TNF-α基因的表达水平上调（P<0.05）。这些结果表明，山羊 CCL5 可能通过调节肺成纤维细胞和巨噬细胞产生的炎性细胞因子在炎症反应中发挥作用。
